The bark of trees is an abundant material for chemical by-products. The combination effects of different concentrations of Acer saccharum var. saccharum inner (IB) and outer (OB) bark acetone extracts with citric acid (CA) applied to Leucaena leucocephala wood were evaluated against the growth of three common molds (Trichoderma viride, Fusarium subglutinans, and Aspergillus niger). IB, OB, and CA solutions were prepared at 0.25% and 0.5% and their combinations were formulated in equal amounts. Acetone extracts of IB and OB were analyzed for their chemicals composition and phenolic compounds using GC/MS and PHLC, respectively. The IB acetone extract contained 4-hydroxy-4-methyl-2-pentanone (31.67%), palmitic acid (15.52%), and linoleic acid (11.14%), while the OB acetone extract contained 1,2-benzenedicarboxylic acid, bis(2-ethylhexyl) ester (9.34%), (Z,E)-9,12-tetradecadien-1-ol (8.86%), and cis-tetrahydro-6-methoxy-2H-pyran-3-ol (5.72%). The HPLC analysis indicated the presence of 14 phenolic compounds in IB extract with major constituents caffeine (362.88 µg/g extract), and p-hydroxy benzoic acid (358.06 µg/g extract), while OB contained 12 compounds with major constituents p-hydroxy benzoic acid (8950.5 µg/g extract), gallic acid (5261 µg/g extract) and salicylic acid (572.38 µg/g extract). High total phenolic content in OB (292.67 mg GAE/g) was associated with high antioxidant activity with an IC50 values of 1.77 and 4.14 μg/mL, as measured by DPPH and β-Carotene-linoleic acid, respectively. The combination treatment of IB0.25%+ OB0.25%+CA0.25% produced the highest antifungal effects against growth of T. viride with an inhibition percentage (IP) of 10.37%, IB0.5%+CA0.5% (IP 16.66%) with F. subglutinans, while CA0.5% and OB0.25%, showed IP of 27.77% and 23.70% with A. niger, respectively. The combination effects of IB, OB and CA could be used as biocide agents for preventing mold growth on wood.

中文翻译：

---

评估糖槭（Acer saccharum var. saccharum）的内外树皮提取物与柠檬酸结合对三种常见霉菌生长的影响

树皮是化学副产品的丰富材料。不同浓度的 Acer saccharum var. 的组合效果。针对三种常见霉菌（绿色木霉、Fusarium subglutinans 和 Aspergillus niger）的生长，评估了应用到银合欢木材上的含柠檬酸 (CA) 的蔗糖内 (IB) 和外 (OB) 树皮丙酮提取物。IB、OB 和 CA 溶液按 0.25% 和 0.5% 制备，它们的组合按等量配制。分别使用 GC/MS 和 PHLC 分析了 IB 和 OB 的丙酮提取物的化学成分和酚类化合物。IB 丙酮提取物含有 4-羟基-4-甲基-2-戊酮 (31.67%)、棕榈酸 (15.52%) 和亚油酸 (11.14%)，而 OB 丙酮提取物含有 1,2-苯二甲酸、双(2-乙基己基)酯 (9. 34%)、(Z,E)-9,12-十四碳二烯-1-醇（8.86%）和顺式-四氢-6-甲氧基-2H-吡喃-3-醇（5.72%）。HPLC 分析表明，IB 提取物中存在 14 种酚类化合物，主要成分为咖啡因（362.88 µg/g 提取物）和对羟基苯甲酸（358.06 µg/g 提取物），而 OB 含有 12 种主要成分为对羟基的化合物苯甲酸（8950.5 µg/g 提取物）、没食子酸（5261 µg/g 提取物）和水杨酸（572.38 µg/g 提取物）。OB 中的高总酚含量 (292.67 mg GAE/g) 与高抗氧化活性相关，IC50 值分别为 1.77 和 4.14 μg/mL，分别由 DPPH 和 β-胡萝卜素-亚油酸测量。IB0.25%+ OB0.25%+CA0.25% 的组合处理对绿色木霉生长产生最高的抗真菌作用，抑制百分比 (IP) 为 10.37%，IB0.5%+CA0.5%（知识产权 16。66%) 与 F. subglutinans，而 CA0.5% 和 OB0.25%，显示 IP 分别为 27.77% 和 23.70% 与 A. niger。IB、OB 和 CA 的组合作用可用作防止木材上霉菌生长的杀菌剂。