Abstract

Candida is a common yeast in opportunistic fungal diseases around the world and is usually colonized on the skin and mucosal membranes. The purpose of this study was to synthesize chitosan–silver–copper nanocomposite and to investigate its antifungal effects on Candida albicans. Silver, copper and chitosan nanoparticles were synthesized individually. Then, copper–silver–chitosan nanocomposite was synthesized. These nanoparticles are approved by transmission electron microscope, and nanocomposite structure was also confirmed by scanning electron microscope. Then, the minimum inhibitory concentrations and minimum fungicidal of these nanostructures were examined on C. albicans. The results of this study indicate that the properties and effects of the investigated nanocomposite are comparable to amphotericin B as standard material. The results show that this effect was higher for copper–silver–chitosan nanocomposite than for other nanoparticles studied. Antifungal effect of copper nanoparticles and chitosan nanoparticles was not established separately, but it was found that their composition had antifungal effects that were effective. The combination of nanoparticles of chitosan with silver has been shown to have some antifungal effects. The most antifungal effect for the nanoparticles studied is related to copper–silver–chitosan nanocomposite and, which has had a significant effect on the growth of C. albicans in the laboratory environment compared to other nanoparticles.

Graphic abstract

中文翻译：

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壳聚糖-银-铜纳米复合材料对白色念珠菌的抗菌作用

摘要

念珠菌是世界上机会性真菌病中的常见酵母，通常定植在皮肤和粘膜上。这项研究的目的是合成壳聚糖-银-铜纳米复合材料，并研究其对白色念珠菌的抗真菌作用。银，铜和壳聚糖纳米粒子分别合成。然后，合成了铜-银-壳聚糖纳米复合材料。这些纳米颗粒通过透射电子显微镜确认，并且通过扫描电子显微镜确认了纳米复合结构。然后，在白色念珠菌上检查了这些纳米结构的最小抑菌浓度和最小杀真菌活性。。这项研究的结果表明，所研究的纳米复合材料的特性和作用与标准的两性霉素B相当。结果表明，铜-银-壳聚糖纳米复合材料的这种作用高于其他研究的纳米颗粒。铜纳米颗粒和壳聚糖纳米颗粒的抗真菌作用没有单独确定，但是发现它们的组成具有有效的抗真菌作用。壳聚糖纳米粒子与银的结合已显示具有一定的抗真菌作用。所研究的纳米颗粒的最大抗真菌作用与铜-银-壳聚糖纳米复合材料有关，与其他纳米颗粒相比，在实验室环境中对白色念珠菌的生长具有显着影响。

图形摘要