Background

Ayurvedic medicines show great promise due to their holistic approach in the treatment of diseases. However, proper standardization is necessary for their integration into mainstream medicine. One such well-known Ayurvedic trailing herb is Benincasa hispida (Thunb.) Cogn. Its fruit contains numerous secondary metabolites, including quercetin, and is used to treat urinary calculi, blood disease, insanity, epilepsy, jaundice, dyspepsia, fever, and menstrual disorders.

Objective

The current investigation was undertaken to develop and validate a rapid, sensitive, and reproducible method for quantifying quercetin in the hydroalcoholic extract of B. hispida fruit pulp (HABH).

Materials and methods

The pre-coated thin-layer chromatography (TLC) aluminum plates with silica gel 60 F254 were used with solvent system comprising toluene–ethyl acetate–formic acid (5:4:0.2, V/V). Determination and quantification were performed by densitometric scanning using a deuterium lamp in the absorbance mode at 262 nm. The validation of precision, accuracy, and reproducibility of the developed high-performance thin-layer chromatographic (HPTLC) method were done as per the International Conference on Harmonization (ICH) guidelines.

Results

The mobile phase used for the development of HPTLC/TLC plate yields a distinct band for quercetin (RF = 0.392). The limit of detection and limit of quantification for the method were found to be 20 and 60 ng per band, respectively. The quantified quercetin content was found to be 193.77 ± 2.86 μg in 10 mg of HABH, i.e., 1.94% w/w of HABH.

Conclusion

This HPTLC method can be successfully employed for the standardization and quantitative analysis of quercetin in formulation containing B. hispida fruit pulp and it will be helpful in the quality control/assurance of such formulations.

中文翻译：

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一种快速高效薄层色谱法来估算组培贝氏菌（槲草）中的槲皮素。水果浆

背景

阿育吠陀药物由于其在疾病治疗中的整体方法而显示出巨大的希望。但是，将它们整合到主流医学中需要适当的标准化。这样的一种著名的印度草药拖尾草药是Benincasa hispida（Thunb。）Cogn。其果实含有大量的次级代谢产物，包括槲皮素，可用于治疗尿结石，血液疾病，精神错乱，癫痫，黄疸，消化不良，发烧和月经紊乱。

目的

目前的调查进行开发和验证快速，灵敏，重现方法中的水醇提取量化槲皮素B.刚毛果肉（HABH）。

材料和方法

带有硅胶60 F254的预涂薄层色谱（TLC）铝板与溶剂体系一起使用，该体系包含甲苯-乙酸​​乙酯-甲酸（5：4：0.2，V / V）。使用氘灯在262 nm处的吸光度模式通过光密度扫描进行测定和定量。按照国际协调会议（ICH）指南对开发的高性能薄层色谱（HPTLC）方法的精度，准确性和可重复性进行验证。

结果

用于开发HPTLC / TLC板的流动相产生槲皮素的独特谱带（R F = 0.392）。该方法的检测限和定量限分别为每条带20 ng和60 ng。发现在10 mg HABH中量化的槲皮素含量为193.77±2.86μg，即HABH为1.94％w / w。

结论

该HPTLC方法可以成功地用于含棉双歧杆菌果肉配方中槲皮素的标准化和定量分析，这将有助于此类配方的质量控制/保证。