Direct resolution of enantiomers of (RS)-ketorolac was achieved by thin-layer chromatography on silica gel plates using enantiomerically pure L-tryptophan, L-valine, L-methionine, and L-histidine as chiral additive in the stationary phase. The solvent system (acetonitrile, methanol water, and chloroform) with different ratios was successful in resolving the enantiomers. Spots were detected by use of iodine vapor. The detection limit was 0.4 μg mL−1 for each enantiomer of (RS)-ketorolac. The native enantiomers were isolated and characterized.

中文翻译：

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固定相中使用L-氨基酸作为手性添加剂的（RS）-酮咯酸的薄层色谱对映体

（RS）-酮咯酸对映体的直接拆分是通过在硅胶板上进行薄层色谱法进行的，该色谱使用对映体纯的L-色氨酸，L-缬氨酸，L-蛋氨酸和L-组氨酸作为固定相中的手性添加剂。不同比例的溶剂体系（乙腈，甲醇水和氯仿）成功拆分了对映异构体。通过使用碘蒸气检测斑点。（RS）-酮咯酸的每个对映异构体的检出限为0.4μgmL -1 。分离和表征天然对映体。