A new, simple, accurate, and precise high-performance thin-layer chromatographic (HPTLC) method has been developed for the separation and simultaneous estimation of aspirin and omeprazole. Standard and sample solutions of aspirin and omeprazole were applied to aluminum foil silica gel G 60 F254 HPTLC plates. The plates were developed with ethyl acetate–dichloromethane–glacial acetic acid 8:2:0.1 (V/V) as the solvent system. HPTLC detection was performed densitometrically at 241 nm. The linear range was 160–960 ng per band for aspirin and 80–480 ng per band for omeprazole, and the correlation coefficients (R2) were 0.991 and 0.998, respectively. The limits of quantification (LOQ) were 31.89 and 8.01 ng per band, respectively, and the respective limits of detection (LOD) were 10.53 and 2.64 ng per band. Analysis of standard solutions revealed that precision and repeatability were good for both compounds studied.

中文翻译：

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高效薄层色谱法同时测定阿司匹林和奥美拉唑

已开发出一种新的，简单，准确和精确的高性能薄层色谱（HPTLC）方法，用于分离和同时估算阿司匹林和奥美拉唑。将阿司匹林和奥美拉唑的标准溶液和样品溶液应用于铝箔硅胶G 60 F254 HPTLC板上。该板用乙酸乙酯-二氯甲烷-冰醋酸8：2：0.1（V / V）作为溶剂体系显影。HPTLC检测在241 nm处进行光密度测定。阿司匹林的线性范围为每条带160–960 ng，奥美拉唑的线性范围为每条带80–480 ng，相关系数（R2）分别为0.991和0.998。定量限（LOQ）分别为每条带31.89和8.01 ng，相应的检测限（LOD）为每条带10.53和2.64 ng。标准溶液的分析表明，所研究的两种化合物的精密度和重复性均良好。