A sensitive, reliable and rapid high-performance thin-layer chromatography (HPTLC) method for the determination of arctiin and arctigenin in Arctium tomentosum Mill. was established. A. tomentosum Mill. extract was used for chromatographic analysis. The ratio of chloroform and methanol was 48:5 as mobile phase. Temperature is 20–23 °C and humidity is less than 30%. The scanning wavelength is 280 nm. The results showed that arctiin had a good linear relationship in the range of 0.5315–5.8465 μg, r = 0.9982; arctigenin had a good linear relationship in the range of 0.5654–6.2194 μg, r = 0.9951. Precision analysis showed that the RSD < 3.0%. The stability study showed that the sample was stable within 24 h at room temperature, RSD < 2.0%. The average recoveries were 103.07 ± 1.57% and 98.55 ± 2.71%, respectively. The antioxidant activity of Arctium tomentosum Mill. was also identified. The results showed that the antioxidant component identified by thin-layer chromatography–1, 1-diphenyl-2-trinitrophenylhydrazine (TLC-DPPH) was arctigenin not arctiin. The proposed HPTLC is a simple and accurate method for the qualitative and quantitative analysis of arctiin and arctigenin in Arctium tomentosum Mill. from different areas.

中文翻译：

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定性和定量分析牛毛草中牛arc苷和牛and苷元的含量。通过高效薄层色谱

一种灵敏，可靠，快速的高效薄层色谱法（HPTLC），用于测定绒毛Arctium Mill中的Arctiin和Arctigenin 。建立了。毛茸茸磨。提取物用于色谱分析。流动相的氯仿与甲醇之比为48：5。温度为20–23°C，湿度小于30％。扫描波长是280nm。结果表明，牛ti苷在0.5315–5.8465μg范围内具有良好的线性关系，r = 0.9982；牛ti皂苷元在0.5654–6.2194μg范围内具有良好的线性关系，r = 0.9951。精密度分析表明，RSD <3.0％。稳定性研究表明，样品在室温下24小时内稳定，RSD <2.0％。平均回收率分别为103.07±1.57％和98.55±2.71％。牛Arc子的抗氧化活性磨。也被确定。结果表明，薄层色谱法鉴定的抗氧化剂成分为1-二苯基-2-三硝基苯肼（TLC-DPPH）是arctigenin而不是arctiin。所提出的HPTLC是牛蒡子苷和牛蒡子苷元的定性和定量分析的简单和准确的方法毛头牛蒡厂。来自不同地区