ABSTRACT Over the last decade, the spectroscopy of cryogenically cold ions isolated in the gas phase has been developed as a new tool for structural elucidations of biological molecules. Cooling allows for vibrational resolution in UV and IR spectra of small to midsize peptides, enabling different multi-laser techniques of conformer-specific spectroscopy. In conjunction with quantum chemistry calculations, IR spectra of single conformers allows for solving their intrinsic geometries. Here, we briefly review some fundamental and technical aspects of the cold-ion spectroscopy (CIS) approach and illustrate its application for protonated peptides, carbohydrates and for non-covalent complexes of biomolecules. The challenges and limitations of CIS in view of its relevance to life-science studies are critically assessed. Finally, we discuss and illustrate some approaches of CIS for the analytical identification of biomolecules, in particular the recently developed method of 2D UV-MS fingerprinting, which combines CIS with high-resolution mass spectrometry.

中文翻译：

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用于生物分子结构鉴定的冷离子光谱

摘要 在过去的十年中，气相中分离出的低温冷离子光谱已发展成为生物分子结构解析的新工具。冷却可以在中小肽的 UV 和 IR 光谱中实现振动分辨率，从而实现构象异构体特异性光谱的不同多激光技术。结合量子化学计算，单个构象异构体的红外光谱可以解决它们的内在几何结构。在这里，我们简要回顾了冷离子光谱 (CIS) 方法的一些基本和技术方面，并说明了其在质子化肽、碳水化合物和生物分子非共价复合物中的应用。鉴于其与生命科学研究的相关性，CIS 的挑战和局限性得到了批判性评估。最后，