Abstract Collagen-chitosan (COL-CS) membranes materials without a cross-linking agent have poor mechanical properties. In this paper, COL-CS membranes were modified by a novel naturally-derived crosslinker, alginate dialdehyde (ADA) with different oxidation degree, and the COL-CS-ADA films were obtained. COL-CS-ADA films were characterized by Fourier transform attenuation total reflection infrared spectroscopy (ATR-FTIR), differential calorimetric scanning (DSC), thermogravimetric analysis (TG), tensile testing, and cross-link density testing. The modification of ADA exhibited positive effects on mechanical properties, the thermal stability of COL-CS membranes. The cross-linking degree between ADA and COL-CS membranes increased significantly with an increase in the oxidation degree. COL-CS-ADA films showed no cytotoxicity toward L929 fibroblasts and had good biocompatibility. The animal experiments showed that COL-CS-ADA film could promote wound healing.

中文翻译：

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氧化海藻酸钠对胶原-壳聚糖膜的改性及用于伤口敷料的体内/体外评价

摘要 不含交联剂的胶原-壳聚糖（COL-CS）膜材料机械性能较差。在本文中，COL-CS 膜被一种新型的天然交联剂、不同氧化程度的海藻酸二醛 (ADA) 改性，获得了 COL-CS-ADA 膜。COL-CS-ADA 薄膜通过傅里叶变换衰减全反射红外光谱 (ATR-FTIR)、差示量热扫描 (DSC)、热重分析 (TG)、拉伸测试和交联密度测试进行表征。ADA 的改性对 COL-CS 膜的机械性能和热稳定性表现出积极的影响。ADA和COL-CS膜之间的交联度随着氧化度的增加而显着增加。COL-CS-ADA 膜对 L929 成纤维细胞没有细胞毒性，具有良好的生物相容性。动物实验表明COL-CS-ADA膜能促进伤口愈合。