Abstract

An approach to the analysis of intracellular motion with nanosecond temporal resolution, based on the Mössbauer study of particles incorporated into a cell, has been developed. A possibility of applying magnetic nanoparticles as probes for studying in vitro the cytoplasm properties is analyzed. Two types of aqueous colloids of nanoparticles enriched with the ⁵⁷Fe isotope are synthesized, and their cytotoxicity and biodegradation resistance under living cell conditions are investigated by an example of breast cancer cell line. It is found that the particles barely undergo any changes characteristic of biodegradation processes during their 120-h incubation in cells, although intracellular oxidation of divalent iron in the particle composition is observed. This result indicates possibility of using these particles for in vitro Mössbauer studies.

中文翻译：

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设计用于研究细胞质粘弹性的磁性纳米粒子的合成和体外抗生物降解性研究

摘要

基于Mössbauer对掺入细胞的颗粒的研究，已开发出一种以纳秒级时间分辨率分析细胞内运动的方法。分析了将磁性纳米粒子用作研究体外细胞质特性的探针的可能性。富含^57的两种纳米颗粒的水性胶体合成了Fe同位素，并以一个乳腺癌细胞系为例研究了它们在活细胞条件下的细胞毒性和抗生物降解性。尽管在颗粒组合物中观察到二价铁的细胞内氧化，但是发现颗粒在其在细胞中120h温育期间几乎没有经历生物降解过程的任何变化特征。该结果表明将这些颗粒用于体外莫斯鲍尔研究的可能性。