Journal of Mass Spectrometry and Advances in the Clinical Lab ( IF 2.1 ) Pub Date : 2020-01-07 , DOI: 10.1016/j.clinms.2019.12.001 Richard B. van Breemen
As in drug discovery and development, mass spectrometry has become essential at all stages for establishing the safety and efficacy of botanical dietary supplements. Applications of mass spectrometry to the development of botanical dietary supplements include preclinical studies of the mechanisms of action (e.g., proteomic target identification and validation); identification of active natural products using high resolution tandem mass spectrometry; chemical standardization using UHPLC-MS/MS; and studies of metabolism, absorption and toxicity of active compounds using high resolution and UHPLC-MS/MS. Clinical applications of mass spectrometry include evaluation of the potential for drug-botanical interactions; investigation of the pharmacokinetics of active compounds; and quantitative analysis of biomarkers of efficacy during Phase I and II and clinical trials of safety and efficacy of botanical dietary supplements.
中文翻译:
质谱在建立植物性膳食补充剂安全性和有效性中的作用
如同在药物发现和开发中一样,质谱仪对于确立植物性膳食补充剂的安全性和有效性在所有阶段都变得至关重要。质谱在植物性膳食补充剂开发中的应用包括作用机理的临床前研究(例如,。,蛋白质组学目标识别和验证);使用高分辨率串联质谱法鉴定活性天然产物;使用UHPLC-MS / MS进行化学标准化;并使用高分辨率和UHPLC-MS / MS研究活性化合物的代谢,吸收和毒性。质谱的临床应用包括评估药物-植物相互作用的潜力;研究活性化合物的药代动力学;I和II期有效性的生物标志物的定量分析和植物性膳食补充剂安全性和有效性的临床试验。