Endometriosis is a recurrent and benign gynecological disorder, defined by the ectopic presence of endometrium. About 10% of reproductive-aged women suffer from endometriosis. There are no non-invasive or minimally invasive tests available in clinical practice to accurately diagnose endometriosis today.

Here, we present our efforts to determine the diagnostic accuracy of biomarkers in peritoneal fluid and blood plasma using flow injection analysis with electrospray ionization tandem mass spectrometry (ESI-MS/MS) in 70 women with endometriosis and 20 women from a control group. The presence of endometriosis was confirmed by surgical findings and post-operative pathological examination. A qualitative and quantitative evaluation of the lipids in peritoneal fluids and blood plasma was carried out using electrospray ionization mass spectrometry (ESI-MS). The analysis revealed more than 140 molecular species of lipids, most of which pertained to five classes: phosphatidylcholines, phosphatidylethanolamines, sphingomyelins, di- and triglycerides. The data were analyzed using a statistical multifactorial method (i.e., PLS-DA). It was found that 9 potential biomarkers of endometriosis (LPC 16:0, PE O-20:0, PE O 34:1, PC 36:2, PC 36:4, PC 36:5, PC 38:4, PC 38:6 and SM 34:1) are common in blood plasma and peritoneal fluid, supporting connection with the pathological process. The sensitivity of the method developed for plasma was 93% with a specificity of 95%; for peritoneal fluid, the sensitivity was 90% and the specificity 95%. Accordingly, plasma is the most suitable biological fluid for clinical diagnostics of endometriosis. Further validation of these lipids as serologic biomarkers may enhance non-invasive diagnostic tools for patients with suspected endometriosis and reduce the frequency of diagnostic laparoscopy.

子宫内膜异位症是一种复发性良性妇科疾病，定义为子宫内膜异位存在。大约 10% 的育龄妇女患有子宫内膜异位症。目前临床实践中没有可用的无创或微创测试来准确诊断子宫内膜异位症。

在这里，我们展示了我们在 70 名子宫内膜异位症女性和 20 名对照组女性中使用流动注射分析和电喷雾电离串联质谱 (ESI-MS/MS) 确定腹膜液和血浆中生物标志物诊断准确性的努力。手术结果和术后病理检查证实了子宫内膜异位症的存在。使用电喷雾电离质谱 (ESI-MS) 对腹膜液和血浆中的脂质进行定性和定量评估。分析揭示了超过 140 种脂质分子，其中大部分属于五类：磷脂酰胆碱、磷脂酰乙醇胺、鞘磷脂、甘油二酯和甘油三酯。使用统计多因素方法（即PLS-DA）分析数据。发现子宫内膜异位症的 9 个潜在生物标志物 (LPC 16:0, PE O-20:0, PE O 34:1, PC 36:2, PC 36:4, PC 36:5, PC 38:4, PC 38 :6 和 SM 34:1) 在血浆和腹腔液中很常见，支持与病理过程的联系。为血浆开发的方法的灵敏度为 93%，特异性为 95%；对于腹腔积液，敏感性为90%，特异性为95%。因此，血浆是最适合临床诊断子宫内膜异位症的生物液体。进一步验证这些脂质作为血清学生物标志物可能会增强对疑似子宫内膜异位症患者的非侵入性诊断工具，并减少诊断性腹腔镜检查的频率。1)常见于血浆和腹腔液，与病理过程有支持联系。为血浆开发的方法的灵敏度为 93%，特异性为 95%；对于腹腔积液，敏感性为90%，特异性为95%。因此，血浆是最适合临床诊断子宫内膜异位症的生物液体。进一步验证这些脂质作为血清学生物标志物可能会增强对疑似子宫内膜异位症患者的非侵入性诊断工具，并减少诊断性腹腔镜检查的频率。1)常见于血浆和腹腔液，与病理过程有支持联系。为血浆开发的方法的灵敏度为 93%，特异性为 95%；对于腹腔积液，敏感性为90%，特异性为95%。因此，血浆是最适合临床诊断子宫内膜异位症的生物液体。进一步验证这些脂质作为血清学生物标志物可能会增强对疑似子宫内膜异位症患者的非侵入性诊断工具，并减少诊断性腹腔镜检查的频率。血浆是最适合临床诊断子宫内膜异位症的生物液体。进一步验证这些脂质作为血清学生物标志物可能会增强对疑似子宫内膜异位症患者的非侵入性诊断工具，并减少诊断性腹腔镜检查的频率。血浆是最适合临床诊断子宫内膜异位症的生物液体。进一步验证这些脂质作为血清学生物标志物可能会增强对疑似子宫内膜异位症患者的非侵入性诊断工具，并减少诊断性腹腔镜检查的频率。