Here, volumetric absorptive microsampling (VAMS), used for the measurement of cardiovascular drugs, is compared against conventional dried blood spot (DBS) card sampling to evaluate adherence to prescribed medication. Volumetric absorptive microsampling (VAMS) is an attractive alternative to plasma sampling for routine drug monitoring and potentially overcomes haematocrit issues associated with quantitative bioanalysis of conventional dried blood spots. A quantitative VAMS-based LC-HRAM MS assay for atenolol, lisinopril, simvastatin and valsartan was developed and validated. The assay demonstrated acceptable linearity, selectivity, accuracy, precision, recovery and insignificant matrix effects with no impact of haematocrit on assay accuracy. Volunteers provided both VAMS and DBS 903 card samples (the current standard) to allow comparison of the two methods and demonstrate the potential utility of VAMS. Analysis of VAMS samples correctly identified drugs in volunteers known to be adherent, and found no false positives from volunteers known to be taking no medication. There was a strong correlation between the two sampling systems confirming the utility of VAMS. Therapeutic drug monitoring (TDM) can assist clinicians in deciding how to proceed with treatment in the event of poor improvement in patient health. VAMS could offer a potentially more efficient method of sample collection, with fewer rejected samples than the DBS approach.

在此，将用于测量心血管药物的容积吸收微量采样（VAMS）与常规干血斑（DBS）卡采样进行比较，以评估对处方药物的依从性。容积吸收微量采样（VAMS）是常规血浆监测中血浆采样的一种有吸引力的替代方法，它有可能克服与常规干血斑定量生物分析相关的血细胞比容问题。开发并验证了基于VAMS的定量方法，用于阿替洛尔，赖诺普利，辛伐他汀和缬沙坦的LC-HRAM MS分析。该测定法显示出可接受的线性，选择性，准确性，精确度，回收率和微不足道的基质效应，而血细胞比容对测定法准确性没有影响。志愿者提供了VAMS和DBS 903卡样本（当前标准），以便比较这两种方法并证明VAMS的潜在用途。对VAMS样品的分析正确地识别了已知依从的志愿者中的药物，并且未发现已知未服用药物的志愿者有误报。两种采样系统之间具有很强的相关性，从而确认了VAMS的实用性。在患者健康状况不佳的情况下，治疗药物监测（TDM）可以帮助临床医生决定如何进行治疗。VAMS可以提供​​一种可能更有效的样本收集方法，与DBS方法相比，拒绝的样本更少。并没有发现已知没有服用药物的志愿者有误报。两种采样系统之间具有很强的相关性，从而确认了VAMS的实用性。在患者健康状况不佳的情况下，治疗药物监测（TDM）可以帮助临床医生决定如何进行治疗。VAMS可以提供​​一种可能更有效的样本收集方法，与DBS方法相比，拒绝的样本更少。并没有发现已知没有服用药物的志愿者有误报。两种采样系统之间具有很强的相关性，从而确认了VAMS的实用性。在患者健康状况不佳的情况下，治疗药物监测（TDM）可以帮助临床医生决定如何进行治疗。VAMS可以提供​​一种可能更有效的样本收集方法，与DBS方法相比，拒绝的样本更少。