Here, volumetric absorptive microsampling (VAMS), used for the measurement of cardiovascular drugs, is compared against conventional dried blood spot (DBS) card sampling to evaluate adherence to prescribed medication. Volumetric absorptive microsampling (VAMS) is an attractive alternative to plasma sampling for routine drug monitoring and potentially overcomes haematocrit issues associated with quantitative bioanalysis of conventional dried blood spots. A quantitative VAMS-based LC-HRAM MS assay for atenolol, lisinopril, simvastatin and valsartan was developed and validated. The assay demonstrated acceptable linearity, selectivity, accuracy, precision, recovery and insignificant matrix effects with no impact of haematocrit on assay accuracy. Volunteers provided both VAMS and DBS 903 card samples (the current standard) to allow comparison of the two methods and demonstrate the potential utility of VAMS. Analysis of VAMS samples correctly identified drugs in volunteers known to be adherent, and found no false positives from volunteers known to be taking no medication. There was a strong correlation between the two sampling systems confirming the utility of VAMS. Therapeutic drug monitoring (TDM) can assist clinicians in deciding how to proceed with treatment in the event of poor improvement in patient health. VAMS could offer a potentially more efficient method of sample collection, with fewer rejected samples than the DBS approach.

在此，将用于测量心血管药物的体积吸收微量采样 (VAMS) 与传统的干血点 (DBS) 卡采样进行比较，以评估对处方药物的依从性。体积吸收微量采样 (VAMS) 是常规药物监测中血浆采样的一种有吸引力的替代方案，并有可能克服与传统干血斑定量生物分析相关的血细胞比容问题。开发并验证了基于 VAMS 的阿替洛尔、赖诺普利、辛伐他汀和缬沙坦的定量 LC-HRAM MS 检测方法。该测定表现出可接受的线性、选择性、准确性、精密度、回收率和微不足道的基质效应，血细胞比容对测定准确性没有影响。志愿者提供了 VAMS 和 DBS 903 卡样本（当前标准），以便比较两种方法并展示 VAMS 的潜在效用。对 VAMS 样本的分析正确地识别了已知依从性志愿者的药物，并且没有发现已知未服用药物的志愿者的假阳性结果。两个采样系统之间存在很强的相关性，证实了 VAMS 的实用性。治疗药物监测 (TDM) 可以帮助临床医生在患者健康状况改善不佳的情况下决定如何继续治疗。 VAMS 可以提供一种潜在更有效的样本收集方法，与 DBS 方法相比，拒绝样本更少。