Multi-wall carbon nanotube (MWNT) was chemically oxidized by utilizing sulfuric and nitric acids in a ratio (2:1 v/v). The oxidized-MWNT was decorated with gold (Au) and gold oxide (Au₂O₃) nanoparticles by using precipitation approach. The Fourier transform infrared (FTIR) and zeta potential techniques verified oxidization of MWNTs with oxygen functional groups. The X-ray diffraction (XRD), ultraviolet–visible (UV–Vis) spectroscopy, and electron microscope were confirmed the coating of Au and Au₂O₃ nanoparticles onto MWNTs surface. The dual florescent assay and colony forming assay were used to assess antibacterial activity against Escherichia coli (E. coli) after incubation in the dark and under visible light exposure. Comparing with the raw-MWNTs and the oxidized-MWNTs, which were investigated to have poor bactericidal ability against E. coli, MWNTs/Au and MWNTs/Au₂O₃ revealed stronger bactericidal efficiency, essentially after photo-activation for 3 h. Moreover, MTT assay showed that the nanohybrids have higher cell viability than raw-MWNTs and the oxidized-MWNTs. The obtained results propose that nanohybrids were prepared as potent antibacterial materials to inhibit and control the persistence and spreading of microorganism diseases.

中文翻译：

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研究金和多壁碳纳米管上装饰的金和氧化金纳米粒子的杀菌能力和生物相容性

多壁碳纳米管（MWNT）通过以2：1 v / v的比例使用硫酸和硝酸进行化学氧化。采用沉淀法对氧化的MWNT进行了金（Au）和氧化金（Au ₂ O ₃）纳米粒子修饰。傅里叶变换红外（FTIR）和Zeta电位技术验证了具有氧官能团的MWNT的氧化。X射线衍射（XRD），紫外-可见（UV-Vis）光谱和电子显微镜证实了Au和Au ₂ O ₃纳米颗粒在MWNT表面上的涂层。双荧光测定和集落形成测定法来评估抗抗菌活性的大肠杆菌（大肠杆菌）在黑暗中和可见光下孵育后。与被调查的对大肠杆菌具有较弱的杀菌能力的原始多壁碳纳米管和氧化多壁碳纳米管相比，MWNTs / Au和MWNTs / Au ₂ O _3表现出更强的杀菌效率，基本上是在光活化3 h后。此外，MTT分析表明纳米杂合体比原始多壁碳纳米管和氧化的多壁碳纳米管具有更高的细胞活力。所得结果表明，制备了纳米杂化物作为有效的抗菌材料，以抑制和控制微生物疾病的持久性和传播。