Abstract In this study, the purified pectin lyase was immobilized in calcium alginate beads and compared with crude enzyme for application in degumming of buel and banana plant fibres. From the data of scanning electron microscopy (SEM), it was observed that untreated buel fibres were covered by non-cellulosic materials (pectin, hemicelluloses and waxes) and the surface of enzymatically treated buel fibres looked smoother. Also, the crude alkaline pectin lyase treated buel fibre exhibited a considerably cleaner surface, which suggested that the crude pectin lyase could provide better degumming effects in comparison to the immobilized pectin lyase. In case of banana fibre, the FTIR spectroscopy showed that both crude and immobilized alkaline pectin lyase treatments of banana plant fibres were equally efficient in degumming. The enzymatic degumming of buel and banana with crude pectin lyase resulted in maximum release of galacturonide after 24 h for buel and 15 h for banana fibre. The optimum temperature for degumming of buel and banana fibres with crude pectin lyase was found to be 50 °C and 45 °C, respectively. Also, the maximum galacturonide was released with 200 and 250 U of pectin lyase for buel and banana fibre, respectively.

中文翻译：

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海藻酸钙固定化和蜡状芽孢杆菌粗果胶裂解酶在植物纤维脱胶中的应用

摘要 在本研究中，将纯化的果胶裂解酶固定在海藻酸钙珠中，并与粗酶进行了比较，以用于布尔和香蕉植物纤维的脱胶。从扫描电子显微镜（SEM）的数据可以看出，未经处理的燃料纤维被非纤维素材料（果胶、半纤维素和蜡）覆盖，经酶处理的燃料纤维表面看起来更光滑。此外，粗碱性果胶裂解酶处理的燃料纤维表现出相当清洁的表面，这表明与固定化果胶裂解酶相比，粗果胶裂解酶可以提供更好的脱胶效果。在香蕉纤维的情况下，FTIR 光谱表明香蕉植物纤维的粗制和固定化碱性果胶裂解酶处理在脱胶方面同样有效。buel 和香蕉用粗果胶裂解酶的酶促脱胶导致 buel 24 小时和香蕉纤维 15 小时后半乳糖醛酸的最大释放。发现用粗果胶裂解酶对布尔纤维和香蕉纤维进行脱胶的最佳温度分别为 50 °C 和 45 °C。此外，对于 buel 和香蕉纤维，分别使用 200 和 250 U 的果胶裂解酶释放了最大的半乳糖醛酸。