Aim: This study evaluated the influence of periodontal therapy on the microbiological profile of individuals with Grade C Molar-Incisor Pattern Periodontitis (C/MIP).

Methods: Fifty-three African-American participants between the ages of 5–25, diagnosed with C/MIP were included. Patients underwent full mouth mechanical debridement with systemic antibiotics (metronidazole 250 mg + amoxicillin 500 mg, tid, 7 days). Subgingival samples were collected from a diseased and a healthy site from each individual prior to treatment and at 3, 6, 12, 18 and 24 months after therapy from the same sites. Samples were subjected to a 16S rRNA gene based-microarray.

Results: Treatment was effective in reducing the main clinical parameters of disease. Aggregatibacter actinomycetemcomitans (A.a.) was the strongest species associated with diseased sites. Other species associated with diseased sites were Treponema lecithinolyticum and Tannerella forsythia. Species associated with healthy sites were Rothia dentocariosa/mucilaginosa, Eubacterium yurii, Parvimonas micra, Veillonella spp., Selenomonas spp., and Streptococcus spp. Overall, treatment was effective in strongly reducing A.a. and other key pathogens, as well as increasing health-associated species. These changes were maintained for at least 6 months.

Conclusions:Treatment reduced putative disease-associated species, particularly A.a., and shifted the microbial profile to more closely resemble a healthy-site profile. (Clinicaltrials.gov registration #NCT01330719).

目的：本研究评估了牙周治疗对C级磨牙-切牙型牙周炎（C / MIP）患者微生物谱的影响。

方法：包括53名5-25岁的被诊断患有C / MIP的非裔美国人。患者使用全身性抗生素（甲硝唑250 mg +阿莫西林500 mg，tid，7天）进行全口机械清创术。在治疗之前以及在治疗后3、6、12、18和24个月从相同部位从每个个体的患病和健康部位采集龈下样品。将样品置于基于16S rRNA基因的微阵列中。

结果：治疗有效地降低了疾病的主要临床参数。放线杆菌聚合酶（Aa。）是与患病部位相关的最强物种。与患病部位有关的其他物种是解脂螺旋体和连翘坦氏菌。与健康相关的网站物种是Rothia dentocariosa / mucilaginosa，真杆菌尤里，Parvimonas MICRA，韦荣球菌属，月形属和链球菌属。总体而言，治疗可有效降低Aa。和其他关键病原体，以及与健康有关的物种不断增加。这些更改至少要维持6个月。

结论：治疗减少了与疾病相关的推定物种，尤其是Aa。，并改变了微生物谱，使其更类似于健康部位。（Clinicaltrials.gov注册号：NCT01330719）。