Oocyte proteins play an important role in oocyte maturation, fertilization and embryonic development. However, the protein composition of mouse germinal vesicle (GV) oocytes is still unclear. Using one‐dimensional Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis (1D SDS‐PAGE) and Reverse‐phase liquid chromatography tandem mass spectrometry (RP‐LC‐MS/MS), we constructed a protein profile of mouse GV oocytes. First, our proteomics profile identified 1,405 different proteins from 11,000 mouse GV oocytes lacking zona pellucida. Second, with detailed bioinformatics analysis, a group of proteins that play an essential role in oocyte maturation was screened. In addition, the expression and localization of suppressor of G2 allele of skp1(SUGT1, also called SGT1), heterogeneous nuclear ribonucleoprotein K (Hnrpk), Seruin, Cullin1(Clu1) and nuclear distribution protein C (Nudc) in mouse ovaries and early embryos were also captured and investigated in this study. Moreover, the protein profile was submitted to the Proteomics Identifications Database (PRIDE) and is available via ProteomeXchange with the identifier PXD014314. Our research provides valuable resources for the study of oocyte proteins and oocyte maturation and helps to clarify the mechanisms of oocyte maturation.

中文翻译：

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基于蛋白质组学的小鼠GV卵母细胞中卵母细胞成熟相关蛋白的鉴定。

卵母细胞蛋白在卵母细胞成熟，受精和胚胎发育中起重要作用。但是，小鼠生小泡（GV）卵母细胞的蛋白质组成仍不清楚。使用一维十二烷基硫酸钠聚丙烯酰胺凝胶电泳（1D SDS-PAGE）和反相液相色谱串联质谱（RP-LC-MS / MS），我们构建了小鼠GV卵母细胞的蛋白质谱。首先，我们的蛋白质组学研究从11,000个缺乏透明带的小鼠GV卵母细胞中鉴定出1,405种不同的蛋白质。其次，通过详细的生物信息学分析，筛选了在卵母细胞成熟中起重要作用的一组蛋白质。此外，skp1（SUGT1，也称为SGT1），异质核糖核蛋白K（Hnrpk），Seruin，G2等位基因的抑制子的表达和定位 这项研究还捕获并研究了小鼠卵巢和早期胚胎中的Cullin1（Clu1）和核分布蛋白C（Nudc）。此外，蛋白质图谱已提交到蛋白质组学鉴定数据库（PRIDE），可通过ProteomeXchange获得，标识为PXD014314。我们的研究为卵母细胞蛋白和卵母细胞成熟的研究提供了宝贵的资源，并有助于阐明卵母细胞成熟的机制。