Separation of X and Y chromosome‐bearing sperm is an appropriate method for the selection of desired sex of offspring to increase the profit in livestock industries. The purpose of this study was the production of a monoclonal antibody against recombinant bovine sex‐determining region Y protein for separation Y sperm. The hybridoma cells from splenocytes of immunized female's balb/C mice and Sp2/0 cells were made. The binding affinity of our monoclonal antibody (mAbSRY2) was compared with mouse monoclonal SRY‐15. The Western blot method indicated that mAbSRY2 successfully detected the rbSRY protein. The specificity and sensitivity of mAbSRY2 is comparable to SRY‐15 commercially ones. The SRY gene in 100% of bull semen contains the Y chromosome that had the strongest binding affinity to mAbSRY2 was synthesized. In other words, the binding affinity of semen contains the X sperms near the negative control. In general, this immunological method can help to separate X from Y sperms. However, the mAbSRY2 is bind to Y‐bearing sexed sperm, but in the future; the sexed sperms need to apply in farms.

中文翻译：

---

抗重组牛性别决定区 Y (SRY) 的单克隆抗体的制备及其与携带 Y 染色体的精子的优先结合


分离携带 X 和 Y 染色体的精子是选择所需后代性别以增加畜牧业利润的适当方法。本研究的目的是生产针对重组牛性别决定区 Y 蛋白的单克隆抗体，用于分离 Y 精子。从免疫雌性balb/C小鼠的脾细胞和Sp2/0细胞中制备杂交瘤细胞。我们的单克隆抗体 (mAbSRY2) 的结合亲和力与小鼠单克隆 SRY-15 进行了比较。 Western blot法表明mAbSRY2成功检测到rbSRY蛋白。 mAbSRY2 的特异性和敏感性与商业 SRY-15 相当。 100%公牛精液中的SRY基因含有与mAbSRY2结合力最强的Y染色体。换句话说，精液中含有X精子的结合亲和力接近阴性对照。一般来说，这种免疫学方法可以帮助分离 X 和 Y 精子。然而，mAbSRY2 可以与携带 Y 型的有性精子结合，但在未来；性精子需要在农场申请。