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Melatonin application differentially modulates the enzymes associated with antioxidative machinery and ascorbate-glutathione cycle during arsenate exposure in indica rice varieties.
Plant Biology ( IF 4.2 ) Pub Date : 2020-09-13 , DOI: 10.1111/plb.13181 S Samanta 1 , A Banerjee 1 , A Roychoudhury 1
中文翻译：
褪黑素的应用在in稻品种砷暴露期间差异调节与抗氧化机制和抗坏血酸-谷胱甘肽循环有关的酶。
更新日期：2020-10-16
Plant Biology ( IF 4.2 ) Pub Date : 2020-09-13 , DOI: 10.1111/plb.13181 S Samanta 1 , A Banerjee 1 , A Roychoudhury 1
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- Arsenic (As) contamination and accumulation in rice is a serious concern causing severe oxidative damage. Melatonin acts as a protective agent in plant defence against multiple abiotic stresses. The mechanism of antioxidant function of melatonin during As stress in rice genotypes is less studied.
- In this study, hydroponically‐grown As‐susceptible (Khitish) and As‐tolerant (Muktashri) rice cultivars, subjected to 150 µm arsenate stress, were supplemented with exogenously applied melatonin (20 µm) to examine the plant defence mechanism.
- Melatonin (Mel) increased root and shoot length, fresh and dry weight, chlorophyll a and b content and activated reducing power and free radical scavenging capacity in both rice cultivars. The role of Mel in the sensitive variety appeared to be more prominent with respect to reduced water saturation deficit by reducing endogenous As and H2O2 accumulation, and enhancing overall antioxidant capacity by imposing reduced requirement of catalase for ROS detoxification, and restoring As‐inhibited activity of glutathione‐S‐transferase, glutathione peroxidase and dehydroascorbate reductase. In contrast, melatonin treatment in the tolerant cultivar required reduced involvement of ascorbate peroxidase to deal with As toxicity, and complemented the stress‐mediated inhibition of guaiacol peroxidase activity. Isozyme profiling also established extensive varietal differences with regard to induction of new isoform(s) by Mel during As treatment.
- This study provides clear insights into mechanistic details of the regulation of antioxidative enzymes by melatonin in contrasting rice genotypes, which may prove helpful in generating As tolerance in susceptible rice varieties grown in marginalized soils, thereby improving crop yield and productivity.
中文翻译：
褪黑素的应用在in稻品种砷暴露期间差异调节与抗氧化机制和抗坏血酸-谷胱甘肽循环有关的酶。
- 水稻中砷（As）的污染和积累是引起严重氧化损伤的严重问题。褪黑素在植物防御多种非生物胁迫中起保护剂的作用。水稻基因型砷胁迫期间褪黑素抗氧化功能的机理研究较少。
- 在这项研究中，对承受150 µm砷酸胁迫的水培水稻（Khitish）和耐旱（Muktashri）水稻品种补充了外源施用的褪黑激素（20 µm），以研究植物防御机制。
- 褪黑素（Mel）增加了两个水稻品种的根和茎长，鲜重和干重，叶绿素a和b的含量，并激活了还原能力和自由基清除能力。在通过减少内源性As和H 2 O 2减少水饱和度不足方面，Mel在敏感品种中的作用似乎更加突出。通过降低过氧化氢酶对ROS解毒的需求量并恢复谷胱甘肽S转移酶，谷胱甘肽过氧化物酶和脱氢抗坏血酸还原酶对As的抑制活性来积累和增强总体抗氧化能力。相反，在耐性品种中进行褪黑素处理需要减少抗坏血酸过氧化物酶的参与以应对As毒性，并补充了胁迫介导的愈创木酚过氧化物酶活性的抑制作用。同工酶分析也确定了在Mel处理As过程中Mel诱导的新同工型的广泛品种差异。
- 这项研究提供了对褪黑激素调节水稻基因型中抗氧化酶的机制细节的清晰见解，这可能证明有助于在边缘化土壤中种植的易感水稻品种中产生As耐性，从而提高作物产量和生产力。
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