The MEROPS website (https://www.ebi.ac.uk/merops) and database was established in 1996 to present the classification and nomenclature of proteolytic enzymes. This was expanded to include a classification of protein inhibitors of proteolytic enzymes in 2004. Each peptidase or inhibitor is assigned to a distinct identifier, based on its biochemical and biological properties, and homologous sequences are assembled into a family. Families in which the proteins share similar tertiary structures are assembled into a clan. The MEROPS classification is thus a hierarchy with at least three levels (protein‐species, family, and clan) showing the evolutionary relationship. Several other data collections have been assembled, which are accessed from all levels in the hierarchy. These include, sequence homologs, selective bibliographies, substrate cleavage sites, peptidase–inhibitor interactions, alignments, and phylogenetic trees. The substrate cleavage collection has been assembled from the literature and includes physiological, pathological, and nonphysiological cleavages in proteins, peptides, and synthetic substrates. In this article, we make recommendations about how best to analyze these data and show analyses to indicate peptidase binding site preferences and exclusions. We also identify peptidases where co‐operative binding occurs between adjacent binding sites.

中文翻译：

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如何使用MEROPS数据库和网站来帮助了解肽酶特异性。

的MEROPS网站（https://www.ebi.ac.uk/merops）和数据库成立于1996年提出的蛋白水解酶的分类和命名。2004年将其扩展到包括蛋白水解酶蛋白抑制剂的分类。根据每种肽酶或抑制剂的生化和生物学特性，每种肽酶或抑制剂都被赋予一个不同的标识符，并且同源序列被组装为一个家族。蛋白质共享相似三级结构的家族被组装成家族。该MEROPS因此，分类是具有至少三个层次（蛋白质物种，家族和家族）的层次结构，显示了进化关系。已经组装了其他几个数据集合，可以从层次结构的所有级别访问这些数据集合。这些包括序列同源物，选择性书目，底物切割位点，肽酶-抑制剂相互作用，比对和系统发育树。底物切割集合已从文献中收集，包括蛋白质，肽和合成底物中的生理，病理和非生理切割。在本文中，我们对如何最好地分析这些数据提出了建议，并给出了表明肽酶结合位点偏好和排除的分析方法。我们还鉴定了在相邻结合位点之间发生合作结合的肽酶。