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Revealing secrets of the enigmatic omega subunit of bacterial RNA polymerase
Molecular Microbiology ( IF 2.6 ) Pub Date : 2020-09-13 , DOI: 10.1111/mmi.14603
Juha Kurkela 1 , Julia Fredman 2 , Tiina A Salminen 2 , Taina Tyystjärvi 1
Affiliation  

The conserved omega (ω) subunit of RNA polymerase (RNAP) is the only nonessential subunit of bacterial RNAP core. The small ω subunit (7 kDa–11.5 kDa) contains three conserved α helices, and helices α2 and α3 contain five fully conserved amino acids of ω. Four conserved amino acids stabilize the correct folding of the ω subunit and one is located in the vicinity of the β′ subunit of RNAP. Otherwise ω shows high variation between bacterial taxa, and although the main interaction partner of ω is always β′, many interactions are taxon‐specific. ω‐less strains show pleiotropic phenotypes, and based on in vivo and in vitro results, a few roles for the ω subunits have been described. Interactions of the ω subunit with the β′ subunit are important for the RNAP core assembly and integrity. In addition, the ω subunit plays a role in promoter selection, as ω‐less RNAP cores recruit fewer primary σ factors and more alternative σ factors than intact RNAP cores in many species. Furthermore, the promoter selection of an ω‐less RNAP holoenzyme bearing the primary σ factor seems to differ from that of an intact RNAP holoenzyme.

中文翻译:

揭示细菌 RNA 聚合酶的神秘欧米茄亚基的秘密

RNA聚合酶 (RNAP) 的保守 omega (ω) 亚基是细菌 RNAP 核心的唯一非必需亚基。小 ω 亚基 (7 kDa–11.5 kDa) 包含三个保守的 α 螺旋,螺旋 α2 和 α3 包含五个完全保守的 ω 氨基酸。四个保守氨基酸稳定 ω 亚基的正确折叠,一个位于 RNAP 的 β' 亚基附近。否则 ω 显示细菌分类群之间的高度变异,尽管 ω 的主要相互作用伙伴总是 β',但许多相互作用是分类群特异性的。ω-less 菌株显示出多效性表型,并且基于体内和体外结果,已经描述了 ω 亚基的一些作用。ω 亚基与 β' 亚基的相互作用对于 RNAP 核心组装和完整性很重要。此外,ω 亚基在启动子选择中起作用,因为在许多物种中,无 ω 的 RNAP 核心比完整的 RNAP 核心募集更少的主要 σ 因子和更多的替代 σ 因子。此外,带有主要 σ 因子的 ω-less RNAP 全酶的启动子选择似乎与完整 RNAP 全酶的不同。
更新日期:2020-09-13
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