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Purification of the phytase enzyme from Lactobacillus plantarum: The effect on pansy growth and macro–micro element content
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-09-12 , DOI: 10.1002/bab.2026 Fazilet Parlakova Karagöz 1 , Yeliz Demir 2 , Merve Şenol Kotan 3 , Atilla Dursun 1 , Şükrü Beydemir 4, 5 , Neslihan Dikbaş 3
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-09-12 , DOI: 10.1002/bab.2026 Fazilet Parlakova Karagöz 1 , Yeliz Demir 2 , Merve Şenol Kotan 3 , Atilla Dursun 1 , Şükrü Beydemir 4, 5 , Neslihan Dikbaş 3
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In the present study, the phytase enzyme was purified from Lactobacillus plantarum with a 3.08% recovery, 9.57-purification fold, and with a specific activity of 278.82 EU/mg protein. Then, the effects of the 5 EU and 10 EU purified phytase was determined on the plant growth, quality, the macro–micro nutrient content of pansy (Viola × wittrockiana), which is of great importance in ornamental plants industry. The research was established under greenhouse conditions with natural light in 2017. The pansy seeds were coated with phytase enzyme solution, sown in a peat environment, and transferred to pots at the seedling period. In general, the 5 EU and 10 EU applications increase plant height, the number of leaves per plant, the number of side branches per plant, and flower height parameters compared to control. Also, micro- and macronutrient values in soil and plant samples were examined. According to the results, the phytase application on pansy cultivation positively affected the properties and yielded high quality of plants.
中文翻译：
从植物乳杆菌中纯化植酸酶：对三色堇生长和宏微量元素含量的影响
在本研究中，植酸酶从植物乳杆菌中纯化，回收率为 3.08%，纯化倍数为 9.57，比活性为 278.82 EU/mg 蛋白质。然后，确定了 5 EU 和 10 EU 纯化植酸酶对三色堇（Viola × wittrockiana)，这在观赏植物行业具有重要意义。该研究于2017年在自然光的温室条件下建立。三色堇种子涂有植酸酶溶液，在泥炭环境中播种，并在苗期转移到盆中。一般而言，与对照相比，5 EU 和 10 EU 应用增加了植物高度、每株植物的叶子数、每株植物的侧枝数和花高参数。此外，还检查了土壤和植物样品中的微量和常量营养素值。根据结果，植酸酶在三色堇栽培中的应用对植物的特性产生了积极的影响，并产生了高质量的植物。
更新日期：2020-09-12
中文翻译：
从植物乳杆菌中纯化植酸酶：对三色堇生长和宏微量元素含量的影响
在本研究中，植酸酶从植物乳杆菌中纯化，回收率为 3.08%，纯化倍数为 9.57，比活性为 278.82 EU/mg 蛋白质。然后，确定了 5 EU 和 10 EU 纯化植酸酶对三色堇（Viola × wittrockiana)，这在观赏植物行业具有重要意义。该研究于2017年在自然光的温室条件下建立。三色堇种子涂有植酸酶溶液，在泥炭环境中播种，并在苗期转移到盆中。一般而言，与对照相比，5 EU 和 10 EU 应用增加了植物高度、每株植物的叶子数、每株植物的侧枝数和花高参数。此外，还检查了土壤和植物样品中的微量和常量营养素值。根据结果，植酸酶在三色堇栽培中的应用对植物的特性产生了积极的影响，并产生了高质量的植物。
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