A new 4-substituted pyrene derivative 4,4,5,5-tetramethyl-2-(4-((pyren-4- yloxy)methyl)phenyl)-1,3,2-dioxaborolane (4-PYB) has been designed and synthesized by introducing phenyl boronic ester to the precursor 4-hydroxylpyrene. Compound 4-PYB with large Stokes shift displays relatively outstanding sensitivity and selectivity for H₂O₂, which is successfully applied to detect H₂O₂ in living cells. The fluorescent properties of probe 4-PYB displays significantly different properties from its derivative Py-Boe based on 1-hydroxylpyrene, further indicating the functionalization of pyrene on the different position is still an effective strategy to develop novel pyrene-based functional materials.

设计了一种新的4-取代pyr衍生物4,4,5,5-四甲基-2-（4-（（（pyren- 4- yloxy）methyl）phenyl）-1,3,2-dioxaborolane（4-PYB）并通过将苯基硼酸酯引入前体4-羟基lp合成。具有大斯托克斯位移的化合物4-PYB对H ₂ O ₂表现出相对出色的灵敏度和选择性，已成功应用于检测活细胞中的H ₂ O ₂。探针4-PYB的荧光性质与其衍生的Py-Boe表现出明显不同的性质 基于1-羟基lp的化合物，进一步表明of在不同位置的官能化仍然是开发新型novel基功能材料的有效策略。