Fluorescent copper microspheres (CMS) with high quantum yield (72.9 %) were synthesized by an efficient, one-pot, and rapid aggression-induced mechanism. The CMS was bright brick-red luminescent under UV light and have shown an excitation independent emission at 670 nm. The emission intensity of CMS was significantly quenched by the addition of nicotinamide adenine dinucleotide (NADH) when excited at 275 nm, providing a linear range with the logarithmic concentration of NADH, suitable for the detection of low concentrations of NADH. Similarly, the emission intensity at 670 nm was enhanced when excited at 340 nm, providing a linear range for NADH determination in the higher concentration. Thus, a dual-mode sensing platform for the determination of NADH was established by utilizing the fluorescent quenching and enhancement properties of NADH over CMS. Addition of other common interfering molecules and ions induced only a meager change in the fluorescent intensity of CMS, suggesting the high selectivity of the sensor. The linear range observed for NADH by exploring the fluorescent quenching properties was from 24.8−575.2 μM (R² = 0.99) with a L.O.D of 2.4 μM. Meanwhile, the linear range observed for NADH by utilizing the fluorescent enhancing properties was from 625 μM-2.30 mM (R² = 0.98) with a L.O.D of 322.5 μM.

荧光铜微球（CMS）具有高量子产率（72.9％）的合成是通过有效的，一锅式的，快速的侵略性诱导机理来进行的。CMS在紫外光下呈亮砖红色发光，并在670 nm处显示出与激发无关的发射。当在275 nm激发时，通过添加烟酰胺腺嘌呤二核苷酸（NADH）可以显着淬灭CMS的发射强度，从而提供与NADH对数浓度呈线性范围的曲线，适合检测低浓度的NADH。同样，在340 nm激发时，670 nm的发射强度得到增强，从而为较高浓度的NADH测定提供了线性范围。从而，通过利用CMS上NADH的荧光猝灭和增强特性，建立了用于测定NADH的双模传感平台。其他常见干扰分子和离子的添加​​仅引起CMS荧光强度的微弱变化，表明传感器具有很高的选择性。通过探索荧光猝灭特性观察到的NADH的线性范围为24.8−575.2μM（R²  = 0.99）的LOD为2.4μM。同时，利用荧光增强特性观察到的NADH的线性范围为625μM-2.30mM（R ²  = 0.98），LOD为322.5μM。