Clinical therapeutic and immunoregulatory effects of recombinant SPLUNC1 protein (rSPLUNC1) were evaluated in Mycoplasma ovipneumoniae (Mo)-infected Argali hybrid sheep (AHS). Group A contained six Bashibai sheep (BS) and groups B–D contained six AHS each. All sheep were manually infected with Mo. Five days post-infection, rSPLUNC1 from BS and AHS was injected intratracheally into group C and D animals; physiological saline was administered to groups A and B. Serum IL-5, IL-6, and IL-9 were quantified by ELISA. After sacrificing the sheep, lung tissues were extracted for pathological examination. The qPCR was used to quantify Mo load in the lungs and evaluate therapeutic efficacy. Serum IL-5, IL-6, and IL-9 concentrations increased during early infection stages in all groups but were significantly lower in groups A, C, and D than in group B on days 14 and 21. On day 21, IL-5 concentrations were lower in group A than in groups C and D. IL-6 concentration in groups A, C, and D was significantly lower than that in group B, and that in groups C and D was significantly lower than that in group A. Mean mycoplasma pneumonia histopathology scores were significantly lower in groups C and D than in group B, and Mo load in group C and D lung tissue decreased significantly compared to that in group B. Intratracheal injection of rSPLUNC1 into Mo-infected sheep decreased the cytokine levels and alleviated clinical symptoms with no mortality. rSPLUNC1 had significant therapeutic effects on Mo-infected AHS and can regulate pro-inflammatory cytokines.

评估重组SPLUNC1蛋白（rSPLUNC1）在卵巢支原体中的临床治疗和免疫调节作用（Mo）感染的盘羊（AHS）。A组包含六只巴什拜羊（BS），B–D组分别包含六只AHS。感染后第5天，将来自BS和AHS的rSPLUNC1气管内注射到C和D组动物中；在感染后5天。将生理盐水给予A和B组。通过ELISA对血清IL-5，IL-6和IL-9进行定量。处死绵羊后，取出肺组织进行病理检查。qPCR用于定量肺中的Mo负荷并评估治疗效果。所有组在感染初期，血清IL-5，IL-6和IL-9的浓度均升高，但在第14天和第21天，A，C和D组的血清IL-5，IL-6和IL-9浓度显着低于B组。 A组比C组和D组低5个浓度。A，C组，和D明显低于B组，C和D组显着低于A组。C和D组的平均支原体肺炎组织病理学评分显着低于B组，Mo载量与B组相比，C和D肺组织显着减少。向受Mo感染的绵羊气管内注射rSPLUNC1降低了细胞因子水平，减轻了临床症状，无死亡。rSPLUNC1对Mo感染的AHS具有显着的治疗作用，并且可以调节促炎细胞因子。与B组相比，C和D组肺组织中的Mo和Mo负荷显着降低。向受Mo感染的绵羊气管内注射rSPLUNC1降低了细胞因子水平，减轻了临床症状，无死亡。rSPLUNC1对Mo感染的AHS具有显着的治疗作用，并且可以调节促炎细胞因子。与B组相比，C和D组肺组织中的Mo和Mo负荷显着降低。向受Mo感染的绵羊气管内注射rSPLUNC1降低了细胞因子水平，减轻了临床症状，无死亡。rSPLUNC1对Mo感染的AHS具有显着的治疗作用，并且可以调节促炎细胞因子。