Background

We aimed to examine whether combined donor/recipient variants in the leptin receptor (LEPR) and adiponectin (ADIPOQ) genes may affect outcomes in renal transplantation.

Methods

A total of 233 donors and their corresponding 307 recipients were genotyped for LEPR rs1805094, rs1137100 and rs1137101, and ADIPOQ rs1501299 and rs224176. Combined donor/recipient genetic scores were created to investigate associations with delayed graft function (DGF), graft loss and estimated glomerular filtration rate (eGFR).

Results

Recipients whose donors carried variant alleles of LEPR rs1137100 and rs1137101 had lower risk of DGF [OR = 0.48 (0.24–0.97), p = 0.040] and [OR = 0.47 (0.23–0.95), p = 0.035], respectively. In addition, rs1137101 also showed an inverse association with lower incidence of graft loss [OR = 0.44 (0.31–0.97), p = 0.040]. The analysis of genetic scores of donor/recipients showed that again rs1137101 was inversely associated with both outcomes: OR = 0.46 (0.23–0.92), p = 0.029 and OR = 0.45 (0.11–0.81), p = 0.009, respectively. With regard to graft function, the T-allele of ADIPOQ rs1501299 in the donor was related to higher eGFR values (75.26 ± 29.01 vs. 67.34 ± 25.39 ml/min for wild-type grafts, p = 0.012). Higher combined genetic scores in this same polymorphism were also associated with better function (78.33 ± 31.87 vs. 68.25 ± 24.32 ml/min, p = 0.018). Finally, eGFR values were similar between paired kidneys but they were different when comparing grafts with or without the rs1501299 T-variant (77.87 ± 26.50 vs. 69.27 ± 26.73 ml/min, p = 0.016).

Conclusions

Our study has shown for the first time to our knowledge that variants in LEPR and ADIPOQ genes of the donors and/or their combination with those in the recipients may affect the outcome of renal transplantation.

中文翻译：

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瘦素受体和脂联素基因多态性联合供受者基因型对肾移植术后并发症发生率的影响

 背景

我们的目的是检查瘦素受体 ( LEPR ) 和脂联素 ( ADIPOQ ) 基因的供体/受体组合变异是否可能影响肾移植的结果。

 方法

共有 233 名捐赠者及其相应的 307 名接受者进行了LEPR rs1805094、rs1137100 和 rs1137101 以及ADIPOQ rs1501299 和 rs224176 的基因分型。创建联合供体/受体遗传评分，以研究与移植物功能延迟 (DGF)、移植物丢失和估计肾小球滤过率 (eGFR) 的关联。

 结果

供体携带LEPR rs1137100和 rs1137101 变异等位基因的受者，DGF 风险较低 [OR = 0.48 (0.24–0.97), p = 0.040] 和 [OR = 0.47 (0.23–0.95), p = 0.035]。此外，rs1137101 还显示出与较低的移植物丢失发生率呈负相关[OR = 0.44 (0.31–0.97)， p = 0.040]。对供体/受体遗传评分的分析再次表明，rs1137101 与两种结果均呈负相关：分别为 OR = 0.46 (0.23–0.92)， p = 0.029 和 OR = 0.45 (0.11–0.81)， p = 0.009。关于移植物功能，供体中ADIPOQ rs1501299 的 T 等位基因与较高的 eGFR 值相关（野生型移植物为 75.26 ± 29.01 与 67.34 ± 25.39 ml/min， p = 0.012）。同一多态性中较高的综合遗传评分也与更好的功能相关（78.33 ± 31.87 vs. 68.25 ± 24.32 ml/min， p = 0.018）。最后，配对肾脏之间的 eGFR 值相似，但在比较有或没有 rs1501299 T 变体的移植物时，eGFR 值不同（77.87 ± 26.50 与 69.27 ± 26.73 ml/min， p = 0.016）。

 结论

据我们所知，我们的研究首次表明，供体的LEPR和ADIPOQ基因变异和/或它们与受者基因的组合可能会影响肾移植的结果。