Skin is a target for hormones and a site of hormone production. Aromatase inhibitors such as letrozole reduce circulating estrogen. The aim of the study was to investigate the morphology of the dermis and immunoexpression of androgen receptor (AR), estrogen receptor α and β (ERα, ERβ), luteinizing hormone receptor (LHR), follicle-stimulating hormone receptor (FSHR), and cytochrome P450 aromatase (P450arom) in male rats with a deficit of estradiol. Experiments were performed on skin of 12 male rats. Rats in the experimental group received per os letrozole for 6 months. For morphological analysis, van Gieson, Sirius Red and orcein staining of sections was performed. In immunohistochemistry, reactions with specific antibodies (anti-P450arom, LHR, FSHR, ERα, ERβ) were used. In morphometric analysis, sections were stained with hematoxylin and eosin. Differences between groups were assessed by Mann-Whitney U-test. There were no differences in the diameter of collagen fibers. The dermis of letrozole-treated animals showed areas without collagen fibers, and expression of P450arom, ERα and ERβ was diminished in the skin of these animals. This study indicates that estrogens exert an effect via ERs that has a role in maintaining proper skin morphology in males, together with androgen. This is also the first documented expression of FSHR in the skin of male rats.

皮肤是荷尔蒙的靶标，也是荷尔蒙产生的场所。芳香酶抑制剂（如来曲唑）可降低循环雌激素。该研究的目的是研究真皮的形态以及雄激素受体（AR），雌激素受体α和β（ERα，ERβ），促黄体激素受体（LHR），促卵泡激素受体（FSHR）和雌二醇不足的雄性大鼠体内的细胞色素P450芳香化酶（P450arom）。在12只雄性大鼠的皮肤上进行实验。接收在实验组大鼠口服来曲唑6个月。为了进行形态分析，进行了切片的van Gieson，Sirius Red和大蜡素染色。在免疫组织化学中，使用了与特异性抗体（抗P450arom，LHR，FSHR，ERα，ERβ）的反应。在形态分析中，切片用苏木精和曙红染色。通过Mann-Whitney U检验评估组之间的差异。胶原纤维的直径没有差异。经来曲唑处理的动物的真皮显示无胶原纤维的区域，这些动物的皮肤中P450arom，ERα和ERβ的表达减少。这项研究表明，雌激素通过雌激素发挥作用，与雄激素一起可维持男性的适当皮肤形态。这也是FSHR在雄性大鼠皮肤中的第一个文献报道。