Biolistic systems are used to shoot exogenous DNA, RNA, protein, and other macromolecules to transfer them into cells for genetic transformation, genome editing, and drug delivery. Such systems are especially useful for plants and other organisms that are incompatible with other macromolecule delivery methods. Commercially available, conventional biolistic systems consist of a shooting device (or “gun”) and a cylinder bottle for high-pressure helium gas. These cost a lot for installation and have low portability. We assembled an inexpensive air duster gun and a hand pump into a portable tool to shoot genes by a man-made air pressure (TSGAMAP). TSGAMAP allows to shoot DNA-coated gold particles with the 3-MPa maximum air pressure. When DNA with a fluorescent protein gene, GFP, was shot by TSGAMAP into leaf epidermal cells of onion, leaf lettuce, and Chinese cabbage, for all of these species, some cells in all became to exhibit GFP signals. When GFP was shot with another fluorescent protein gene, mCherry, into Chinese cabbage cells, both GFP and mCherry signals were detected in some cells. When a transcription factor gene AoAMS was fused with GFP and shot into Chinese cabbage cells, nuclear-localized GFP signals were detected in some cells. These results suggest that TSGAMAP can be used for protein coexpression and protein subcellular localization analyses. TSGAMAP is a cost-saving and portable tool to shoot DNA and other microparticles into cells. This can expand the use of biolistics in research and education.

中文翻译：

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开发一种通过人造气压射击基因的工具。

生物射弹系统用于拍摄外源DNA，RNA，蛋白质和其他大分子，以将其转移到细胞中进行遗传转化，基因组编辑和药物递送。这样的系统对于与其他大分子递送方法不相容的植物和其他生物特别有用。可商购的常规生物弹射系统由射击装置（或“枪”）和用于高压氦气的气瓶组成。这些安装成本高昂，可移植性低。我们将廉价的除尘器枪和手动泵组装到便携式工具中，以通过人造气压（TSGAMAP）射击基因。TSGAMAP允许以3 MPa的最大气压发射涂有DNA的金颗粒。当TSGAMAP将带有荧光蛋白基因GFP的DNA射入洋葱，生菜的叶表皮细胞中时，和大白菜，对于所有这些物种，全部细胞中都显示出GFP信号。当GFP用另一个荧光蛋白基因mCherry射入大白菜细胞时，在某些细胞中同时检测到GFP和mCherry信号。当转录因子基因AoAMS与GFP融合并注入大白菜细胞中时，在某些细胞中检测到核定位的GFP信号。这些结果表明TSGAMAP可用于蛋白质共表达和蛋白质亚细胞定位分析。TSGAMAP是一种节省成本的便携式工具，可以将DNA和其他微粒发射到细胞中。这可以扩大生物弹药在研究和教育中的使用。进入大白菜细胞后，在某些细胞中同时检测到GFP和mCherry信号。当转录因子基因AoAMS与GFP融合并注入大白菜细胞中时，在某些细胞中检测到核定位的GFP信号。这些结果表明TSGAMAP可用于蛋白质共表达和蛋白质亚细胞定位分析。TSGAMAP是一种节省成本的便携式工具，可以将DNA和其他微粒发射到细胞中。这可以扩大生物弹药在研究和教育中的使用。进入大白菜细胞后，在某些细胞中同时检测到GFP和mCherry信号。当转录因子基因AoAMS与GFP融合并注入大白菜细胞中时，在某些细胞中检测到核定位的GFP信号。这些结果表明TSGAMAP可用于蛋白质共表达和蛋白质亚细胞定位分析。TSGAMAP是一种节省成本的便携式工具，可以将DNA和其他微粒发射到细胞中。这可以扩大生物弹药在研究和教育中的使用。TSGAMAP是一种节省成本的便携式工具，可以将DNA和其他微粒发射到细胞中。这可以扩大生物弹药在研究和教育中的使用。TSGAMAP是一种节省成本的便携式工具，可以将DNA和其他微粒发射到细胞中。这可以扩大生物弹药在研究和教育中的使用。