Fusarium wilt biocontrol using endophytic microorganisms may represent a potentially attractive and environmentally safe alternative since endophytes could better limit disease incidence and severity through inhibition of the systemic fungus progress. Twenty-three endophytic bacterial isolates, naturally associated with Solanum sodomaeum and Solanum bonariense, were evaluated for their ability to control Fusarium wilt of tomato induced by Fusarium oxysporum f. sp. lycopersici (FOL) and to promote plant growth. Selected endophytic isolates were screened in vivo, using the root dipping and the culture substrate drenching methods. The most bioactive isolates were subjected to morphological and biochemical characterization and subsequent identification through 16S rDNA sequencing genes. Seven isolates (Stenotrophomonas maltophilia S23, S24, S26 and S28; Bacillus sp. SV81; Azotobacter chroococcum S11; and Serratia marcescens S14) were found to be the most efficient in reducing disease severity by 82–96% over control. Treatments with these isolates led to a significant enhancement in growth parameters, estimated at 45.5–61 and 24.2–70.5% than the control, in tomato plants infected or not with FOL, respectively. Diffusible and volatile metabolites released from bacterial cultures had significantly limited FOL radial growth. All isolates were positive for indole-3-acetic acid (IAA) production. S. marcescens S14, S. maltophilia S28, and Bacillus sp. SV81 exhibited a positive phosphate solubilization activity. Production of chitinase, protease, pectinase, and hydrogen cyanide were also investigated. This study clearly demonstrated that endophytic bacteria recovered from these 2 Solanum species can be explored as promising biocontrol agents active against FOL and are able to enhance tomato growth.

中文翻译：

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枯萎病的生物控制和番茄生长刺激，使用天然相关联的内生细菌茄sodomaeum和小号。Bonariense植物

使用内生微生物对枯萎病进行生物防治可能代表了一种潜在的有吸引力且对环境安全的替代方法，因为内生菌可以通过抑制全身真菌的生长更好地限制疾病的发生和严重程度。评估了与番茄茄和茄茄自然相关的二十三个内生细菌分离物控制由尖孢镰刀菌f诱导的番茄枯萎病的能力。sp。lycopersici（FOL）并促进植物生长。使用根浸法和培养物浸入法在体内筛选选定的内生分离物。对最具生物活性的分离株进行形态和生化鉴定，然后通过16S rDNA测序基因进行鉴定。七个分离株（嗜麦芽窄食单胞菌S23，S24，S26和S28；芽孢杆菌 SV81; 嗜绿固氮菌S11；沙雷氏菌和粘质沙雷氏菌（Serratia marcescens）（S14）被发现最有效地将疾病严重程度降低了82.96％。这些分离物的处理分别导致感染或未感染FOL的番茄植株的生长参数显着提高，分别比对照高45.5–61和24.2–70.5％。从细菌培养物中释放出的可扩散和挥发性代谢物显着限制了FOL径向生长。所有分离株的吲哚-3-乙酸（IAA）生产均为阳性。marcescens S14，嗜麦芽孢杆菌S28和芽孢杆菌属。SV81表现出正磷酸盐溶解活性。还研究了几丁质酶，蛋白酶，果胶酶和氰化氢的产生。