Background:

MiR-183-5p plays an important role in the pathophysiology of many tumors, while the role of MiR-183-5p in liver cancer is unclear.

Methods:

In this study, quantitative reverse transcription-polymerase chain reaction and Western blotting were used to detect the expression of miR-183-5p in liver cancer cell lines, liver cancer tissues, and normal tissues adjacent to the cancer, and to explore the mechanism of miR-183-5p regulating liver cancer progression. The in vitro effects of miR-183-5p were evaluated by CCK-8, colony formation test, and wound healing test. Various databases were used to predict the target mRNA of miR-183-5p and verified by luciferase report analysis. In addition, the effects of miR-183-5p and its target gene on the survival of patients with liver cancer were also analyzed.

Results:

miR-183-5p was highly expressed in hepatocellular carcinoma cells and tissues, and was related to some clinicopathological features. MiR-183-5p can promote the proliferation and migration of liver cancer cells. Using the bioinformatics database, we proved that miR-183-5p is related to the survival of liver cancer patients. Insulin receptor substrate 1 (IRS1) is a target of miR-183-5p, and luciferase analysis confirmed that miR-183-5p combines with the 3′-untranslated region (3′-UTR) of IRS1.

Conclusion:

The miR-183-5p/IRS1 axis may be a new target for liver cancer research.

中文翻译：

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miR-183-5p 通过靶向 IRS1 促进肝细胞癌的增殖和迁移及其与患者生存的关联。

背景：

MiR-183-5p在许多肿瘤的病理生理中起重要作用，而MiR-183-5p在肝癌中的作用尚不清楚。

方法：

本研究采用定量逆转录-聚合酶链反应和Western blotting检测miR-183-5p在肝癌细胞系、肝癌组织和癌旁正常组织中的表达，探讨其机制。 miR-183-5p 调节肝癌进展。通过CCK-8、集落形成试验和伤口愈合试验评估miR-183-5p的体外作用。使用各种数据库预测miR-183-5p的靶mRNA并通过荧光素酶报告分析验证。此外，还分析了miR-183-5p及其靶基因对肝癌患者生存的影响。

结果：

miR-183-5p在肝癌细胞和组织中高表达，并与一些临床病理特征有关。MiR-183-5p可以促进肝癌细胞的增殖和迁移。利用生物信息学数据库，我们证明了miR-183-5p与肝癌患者的生存有关。胰岛素受体底物 1 (IRS1) 是 miR-183-5p 的靶标，荧光素酶分析证实 miR-183-5p 与 IRS1 的 3'-非翻译区 (3'-UTR) 结合。

结论：

miR-183-5p/IRS1轴可能是肝癌研究的新靶点。