Human pluripotent stem cells (PSCs), including embryonic stem cells and induced pluripotent stem cells, represent powerful tools for disease modeling and for therapeutic applications. PSCs are particularly useful for the study of development and diseases of the nervous system. However, generating in vitro models that recapitulate the architecture and the full variety of subtypes of cells that make the complexity of our brain remains a challenge. In order to fully exploit the potential of PSCs, advanced methods that facilitate the identification of molecular signatures in neural differentiation and neurological diseases are highly demanded. Here, we review the literature on the development and application of digital color-coded molecular barcoding as a potential tool for standardizing PSC research and applications in neuroscience. We will also describe relevant examples of the use of this technique for the characterization of the heterogeneous composition of the brain tumor glioblastoma multiforme.

中文翻译：

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使用数字彩色编码的分子条形码识别神经分化和神经系统疾病中的分子特征

人多能干细胞（PSC），包括胚胎干细胞和诱导性多能干细胞，代表了用于疾病建模和治疗应用的强大工具。PSC对研究神经系统的发育和疾病特别有用。但是，体外产生概括使大脑复杂的细胞的结构和所有亚型的模型仍然是一个挑战。为了充分利用PSC的潜力，迫切需要能够在神经分化和神经系统疾病中促进分子标记鉴定的先进方法。在这里，我们回顾有关数字彩色编码分子条形码的开发和应用的文献，这些文献是标准化PSC研究和神经科学应用的潜在工具。我们还将描述该技术用于表征多形性脑肿瘤胶质母细胞瘤的异质成分的相关实例。