Nonlinear microscopy has evolved over the last few decades to become a powerful tool for imaging and spectroscopic applications in biological sciences. In this study, ${{\rm i}^2}{\rm PIE}$, a novel spectral phase control technique, was implemented in order to compress broad-bandwidth supercontinuum light pulses generated in an all-normal-dispersion (ANDi) photonic crystal fiber (PCF). The technique, based on time-domain ptychography, is demonstrated here in a nonlinear microscopy application for the first time, to the best of our knowledge. The first real-world application of this technique for second-harmonic generation and two-photon excitation fluorescence microscopies in biological samples is presented. We further show that in our implementation, ${{\rm i}^2}{\rm PIE}$ leads to improved contrast and signal-to-noise ratios in the generated images, compared to conventional compression techniques used in nonlinear microscopy.

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广义纯谱相位时域谱图相位重建在非线性显微镜中的应用

在过去的几十年中，非线性显微镜已经发展成为一种用于生物学中成像和光谱应用的强大工具。在这项研究中，实施了一种新颖的光谱相位控制技术$ {{{rm i} ^ 2} {\ rm PIE} $，以便压缩在全正态色散（ANDi）中产生的宽带超连续谱光脉冲。 ）光子晶体光纤（PCF）。据我们所知，这是首次在非线性显微镜应用中演示了基于时域刻印术的技术。介绍了该技术在生物样品中的二次谐波产生和双光子激发荧光显微照相的首次实际应用。我们进一步证明，在我们的实现中，$ {{\ rm i} ^ 2} {\ rm PIE} $ 与非线性显微镜中使用的常规压缩技术相比，可在生成的图像中改善对比度和信噪比。