Abstract

To improve our understanding of the relationship between soil higher fungi (belonging to Ascomycota and Basidiomycota) and Abies koreana, we surveyed A. koreana soil fungal communities in a forest in Mt. Halla, Jeju Island, Korea by next-generation sequencing (Illumina Miseq). To confirm the soil higher fungal communities, we collected two types of soils from a defined plot: soils with dead (AKDTs) and living A. koreana (AKLTs), respectively. Soil fungi were classified into 2 phyla, 19 classes, 64 orders, 133 families, 195 genera, and 229 OTUs (895,705 sequence reads). Nonmetric multidimensional scaling (NMDS) showed significantly different soil higher fungal communities between AKDTs and AKLTs (p < .05). In addition, the saprophyte composition was significantly affected by A. koreana status (p < .05). The proportion of the mycorrhizal Clavulina spp. was different between soils with AKDTs and AKLTs, suggesting that Clavulina spp. may be a crucial soil fungal species influencing A. koreana. This study will lead to a better understanding of the ecological status of A. koreana in Mt. Halla. In addition, this study could be useful for the conservation and management of A. koreana habitats.

摘要

为了提高我们对土壤高等真菌（属于子囊菌门和担子菌门）与韩国冷杉之间关系的理解，我们通过下一代测序（Illumina Miseq）调查了韩国济州岛汉拿山森林中的A. koreana土壤真菌群落). 为了确认土壤中的高等真菌群落，我们从指定的地块收集了两种类型的土壤：分别是死土壤 (AKDTs) 和活的A. koreana (AKLTs)。土壤真菌分为 2 门、19 纲、64 目、133 科、195 属和 229 个 OTU（895,705 个序列读数）。非度量多维尺度 (NMDS) 显示 AKDT 和 AKLT 之间存在显着不同的土壤高等真菌群落 ( p < .05)。此外，腐生菌的组成受A. koreana状态的显着影响 ( p  < .05)。菌根Clavulina spp的比例。具有 AKDT 和 AKLT 的土壤之间存在差异，表明Clavulina spp。可能是影响A. koreana 的重要土壤真菌种类。这项研究将有助于更好地了解汉拿山A. koreana的生态状况。此外，这项研究可能对A. koreana栖息地的保护和管理有用。