ABSTRACT This study used surface imprinting polymerization to synthesize molecularly imprinted polymer on the surface of PAF-45 material (PAF-45@MIP). SEM, FT-IR, static and kinetic adsorption experiments were adopted to investigate the morphology and properties of the MIP. The results showed that the PAF-45@MIP could rapidly absorb histamine with a high adsorption capacity. Using PAF-45@MIP as a biomimetic antibody and Pt nanozyme as a marker, a novel direct competitive biomimetic immunoassay (BIA) method for rapidly detecting histamine in foods was developed. Under optimal conditions, the limit of detection and sensitivity of the BIA method were 0.128 and 8.306 mg/L, respectively. Vinegar spiked with histamine was determined using the BIA method with recoveries ranging from 81.50% to 90.38%. The BIA method was also applied to determine histamine in dried shrimp and soy sauce samples, the results correlating well with those obtained using high-performance liquid chromatography.

中文翻译：

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基于Pt纳米酶和分子印迹聚合物的仿生免疫分析方法检测食品中的组胺

摘要 本研究利用表面印迹聚合在 PAF-45 材料 (PAF-45@MIP) 表面合成分子印迹聚合物。采用SEM、FT-IR、静态和动态吸附实验来研究MIP的形貌和性质。结果表明，PAF-45@MIP 能够快速吸收组胺，具有较高的吸附容量。以PAF-45@MIP为仿生抗体，Pt纳米酶为标志物，开发了一种新型直接竞争仿生免疫分析（BIA）方法，用于快速检测食品中的组胺。在最佳条件下，BIA 方法的检测限和灵敏度分别为 0.128 和 8.306 mg/L。使用 BIA 方法测定添加组胺的醋，回收率范围为 81.50% 至 90.38%。