Abstract

Here, two types of aptamer (specific to E. coli O157:H7 pathogenic bacteria) Cu₃(PO₄)₂ nanocomposites loaded with Fe₃O₄ nanoparticles (aptamer@Fe₃O₄, magnetic separation) and platinum nanoparticles (PtNPs) (aptamer@PtNP) were prepared in house via enzymatic hydrolysis of H₂O₂ to O₂ through a one-step method. In the proposed point-of-care biosensor, these nanocomposites were used as capture and signal probes for the determination of pathogenic E. coli O157:H7 bacteria via hand-held barometers detecting gas pressure signals in a sealed bottle. The pressure signal was linear when the concentration of E. coli O157:H7 was from 10³ to 10⁸ CFU/mL. With the easily prepared dual aptamer-Cu₃(PO₄)₂ nanocomposites and the hand-held barometer signal readout, the proposed point-of-care biosensor was shown to be a potential tool for the determination of pathogenic bacteria in underdeveloped areas.

摘要

在这里，两种类型的适体（特定于大肠杆菌O157：H7致病菌）Cu ₃（PO ₄）₂纳米复合材料，其中负载有Fe ₃ O ₄纳米颗粒（aptamer @ Fe ₃ O ₄，磁分离）和铂纳米颗粒（PtNPs） （适体@ PtNP）在房子经由h的酶水解制备₂ Ò ₂至O ₂通过一步法。在拟定的即时医疗生物传感器中，这些纳米复合材料被用作捕获和信号探针，用于确定病原性大肠杆菌O157：H7细菌通过手持气压计检测密封瓶中的气压信号。当大肠杆菌O157：H7的浓度为10 ³至10 ⁸ CFU / mL时，压力信号呈线性。借助易于制备的双适体-Cu ₃（PO ₄）₂纳米复合材料和手持气压计信号读数，该拟议的即时医疗生物传感器被证明是确定欠发达地区致病细菌的潜在工具。