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Dual Aptamer-Copper (II) Phosphate Nanocomposite-Based Point-of-Care Biosensor for the Determination of Escherichia coli O157:H7 through Pressure Monitoring with a Hand-Held Barometer
Analytical Letters ( IF 1.6 ) Pub Date : 2020-09-11 , DOI: 10.1080/00032719.2020.1817059
Yinghao Zhao 1, 2 , Shengjun Bu 2 , Chengyu Wang 2 , Chengyou Ma 3 , Zhongyi Li 2 , Wenhui Zhang 1 , Jiayu Wan 2
Affiliation  

Abstract

Here, two types of aptamer (specific to E. coli O157:H7 pathogenic bacteria) Cu3(PO4)2 nanocomposites loaded with Fe3O4 nanoparticles (aptamer@Fe3O4, magnetic separation) and platinum nanoparticles (PtNPs) (aptamer@PtNP) were prepared in house via enzymatic hydrolysis of H2O2 to O2 through a one-step method. In the proposed point-of-care biosensor, these nanocomposites were used as capture and signal probes for the determination of pathogenic E. coli O157:H7 bacteria via hand-held barometers detecting gas pressure signals in a sealed bottle. The pressure signal was linear when the concentration of E. coli O157:H7 was from 103 to 108 CFU/mL. With the easily prepared dual aptamer-Cu3(PO4)2 nanocomposites and the hand-held barometer signal readout, the proposed point-of-care biosensor was shown to be a potential tool for the determination of pathogenic bacteria in underdeveloped areas.



中文翻译:

基于双适体铜(II)磷酸盐纳米复合物的护理点生物传感器,通过手持气压计进行压力监测,用于测定大肠杆菌O157:H7

摘要

在这里,两种类型的适体(特定于大肠杆菌O157:H7致病菌)Cu 3(PO 42纳米复合材料,其中负载有Fe 3 O 4纳米颗粒(aptamer @ Fe 3 O 4,磁分离)和铂纳米颗粒(PtNPs) (适体@ PtNP)在房子经由h的酶水解制备2 Ò 2至O 2通过一步法。在拟定的即时医疗生物传感器中,这些纳米复合材料被用作捕获和信号探针,用于确定病原性大肠杆菌O157:H7细菌通过手持气压计检测密封瓶中的气压信号。当大肠杆菌O157:H7的浓度为10 3至10 8 CFU / mL时,压力信号呈线性。借助易于制备的双适体-Cu 3(PO 42纳米复合材料和手持气压计信号读数,该拟议的即时医疗生物传感器被证明是确定欠发达地区致病细菌的潜在工具。

更新日期:2020-09-11
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