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Draft Genome Assembly of the Freshwater Apex Predator Wels Catfish (Silurus glanis) Using Linked-Read Sequencing.
G3: Genes, Genomes, Genetics ( IF 2.1 ) Pub Date : 2020-10-27 , DOI: 10.1534/g3.120.401711
Mikhail Yu Ozerov 1, 2, 3 , Martin Flajšhans 4 , Kristina Noreikiene 5 , Anti Vasemägi 5, 6 , Riho Gross 5
Affiliation  

The wels catfish (Silurus glanis) is one of the largest freshwater fish species in the world. This top predator plays a key role in ecosystem stability, and represents an iconic trophy-fish for recreational fishermen. S. glanis is also a highly valued species for its high-quality boneless flesh, and has been cultivated for over 100 years in Eastern and Central Europe. The interest in rearing S. glanis continues to grow; the aquaculture production of this species has almost doubled during the last decade. However, despite its high ecological, cultural and economic importance, the available genomic resources for S. glanis are very limited. To fulfill this gap we report a de novo assembly and annotation of the whole genome sequence of a female S. glanis. The linked-read based technology with 10X Genomics Chromium chemistry and Supernova assembler produced a highly continuous draft genome of S. glanis: ~0.8Gb assembly (scaffold N50 = 3.2 Mb; longest individual scaffold = 13.9 Mb; BUSCO completeness = 84.2%), which included 313.3 Mb of putative repeated sequences. In total, 21,316 protein-coding genes were predicted, of which 96% were annotated functionally from either sequence homology or protein signature searches. The highly continuous genome assembly will be an invaluable resource for aquaculture genomics, genetics, conservation, and breeding research of S. glanis.



中文翻译:

使用链接阅读测序技术对淡水Apex捕食者的基因组大会草案进行了We鱼(Silurus glanis)的研究。

威尔士cat鱼(Silurus glanis)是世界上最大的淡水鱼类之一。这种顶级捕食者在生态系统稳定中发挥着关键作用,并且代表休闲渔民的标志性奖杯鱼。S. glanis因其优质的无骨肉而也是一个极有价值的物种,在东欧和中欧已栽培了100多年。对饲养S. glanis的兴趣持续增长。在过去十年中,该物种的水产养殖产量几乎翻了一番。但是,尽管其具有很高的生态,文化和经济重要性,但它们仍可用于桑链球菌的基因组资源非常有限。为了弥补这一差距,我们从头开始装配和注释的整个基因组序列的雌性glanis。基于链读技术,具有10X基因组学铬化学和超新星装配体,产生了高度连续的甘蓝链球菌草图基因组:〜0.8Gb装配(支架N 50 = 3.2 Mb;最长的单个支架= 13.9 Mb; BUSCO完整性= 84.2%) ,其中包括313.3 Mb的推定重复序列。总共预测了21,316个蛋白质编码基因,其中96%从序列同源性或蛋白质特征搜索中进行了功能注释。高度连续的基因组组装将是glanis S. glanis的水产养殖基因组学,遗传学,保护和育种研究的宝贵资源。

更新日期:2020-11-06
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