Acquisition of chromosome 1q duplication in parental and genome-edited human-induced pluripotent stem cell-derived neural stem cells results in their higher proliferation rate in vitro and in vivo,Cell Proliferation

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Acquisition of chromosome 1q duplication in parental and genome-edited human-induced pluripotent stem cell-derived neural stem cells results in their higher proliferation rate in vitro and in vivo
Cell Proliferation ( IF 5.9 ) Pub Date : 2020-09-12 , DOI: 10.1111/cpr.12892
Narges Zare Mehrjardi _{1,

2} , Marek Molcanyi ₁ , Firuze Fulya Hatay ₁ , Marco Timmer ₃ , Ebrahim Shahbazi ₂ , Justus P Ackermann ₄ , Stefan Herms _{5,

6} , Stefanie Heilmann-Heimbach ₅ , Thomas F Wunderlich _{4,

7,

8} , Nora Prochnow ₉ , Aiden Haghikia ₉ , Angelika Lampert ₁₀ , Jürgen Hescheler ₁ , Edmund A M Neugebauer ₁₁ , Hossein Baharvand _{2,

12} , Tomo Šarić ₁

Affiliation

Center for Physiology and Pathophysiology, Institute for Neurophysiology, Medical Faculty, University of Cologne, Cologne, Germany.
Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran.
Department of Neurosurgery, University Hospital Cologne, Cologne, Germany.
Center for Molecular Medicine Cologne, University of Cologne, Cologne, Germany.
Department of Genomics, Life & Brain Center, Institute for Human Genetics, University of Bonn, Bonn, Germany.
Department of Biomedicine, Medical Genetics, Research Group Genomics, University Hospital Basel, Basel, Switzerland.
Max Planck Institute for Metabolism Research and Institute for Genetics, University of Cologne, Cologne, Germany.
Cologne Cluster of Excellence in Cellular Stress Responses in Aging-Associated Diseases (CECAD), Cologne, Germany.
Clinic for Neurology, St. Josef-Hospital, Clinic of the Ruhr-University Bochum, Bochum, Germany.
Institute of Physiology, Uniklinik, RWTH Aachen University, Aachen, Germany.
Medizinische Hochschule Brandenburg Theodor Fontane, Campus Neuruppin, Neuruppin, Germany.
Department of Developmental Biology, University of Science and Culture, Tehran, Iran.

Genetic engineering of human‐induced pluripotent stem cell‐derived neural stem cells (hiPSC‐NSC) may increase the risk of genomic aberrations. Therefore, we asked whether genetic modification of hiPSC‐NSCs exacerbates chromosomal abnormalities that may occur during passaging and whether they may cause any functional perturbations in NSCs in vitro and in vivo.

中文翻译：

在亲本和基因组编辑的人类诱导多能干细胞衍生的神经干细胞中获得染色体 1q 重复导致其体外和体内增殖率更高

人类诱导多能干细胞衍生神经干细胞（hiPSC-NSC）的基因工程可能会增加基因组畸变的风险。因此，我们询问了 hiPSC-NSCs 的基因修饰是否会加剧传代过程中可能发生的染色体异常，以及它们是否会在体外和体内引起 NSCs 的任何功能扰动。

更新日期：2020-09-12

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