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Molecular basis of the silkworm mutant rel causing red egg color and embryonic death
Insect Science ( IF 2.9 ) Pub Date : 2020-09-11 , DOI: 10.1111/1744-7917.12871 Jiang-Wen Luo 1 , Er-Xia An 1 , Ya-Ru Lu 1 , Ling Yang 1 , Ting-Ting Gai 1 , Song-Zhen He 1 , Song-Yuan Wu 1 , Hai Hu 1 , Chun-Lin Li 1 , Cheng Lu 1 , Xiao-Ling Tong 1 , Fang-Yin Dai 1
Insect Science ( IF 2.9 ) Pub Date : 2020-09-11 , DOI: 10.1111/1744-7917.12871 Jiang-Wen Luo 1 , Er-Xia An 1 , Ya-Ru Lu 1 , Ling Yang 1 , Ting-Ting Gai 1 , Song-Zhen He 1 , Song-Yuan Wu 1 , Hai Hu 1 , Chun-Lin Li 1 , Cheng Lu 1 , Xiao-Ling Tong 1 , Fang-Yin Dai 1
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The coloration and hatchability of insect eggs can affect individual and population survival. However, few genetic loci have been documented to affect both traits, and the genes involved in regulating these two traits are unclear. The silkworm recessive mutant rel shows both red egg color and embryo mortality. We studied the molecular basis of the rel phenotype formation. Through genetic analysis, gene screening and sequencing, we found that two closely linked genes, BGIBMGA003497 (Bm-re) and BGIBMGA003697 (BmSema1a), control egg color and embryo mortality, respectively. Six base pairs of the Bm-re gene are deleted in its open reading frame, and BmSema1a is expressed at abnormally low levels in mutant rel. BmSema1a gene function verification was performed using RNA interference and clustered randomly interspersed palindromic repeats (CRISPR)/CRISPR-associate protein 9. Deficiency of the BmSema1a gene can cause the death of silkworm embryos. This study revealed the molecular basis of silkworm rel mutant formation and indicated that the Sema1a gene is essential for insect embryo development.
中文翻译：
蚕突变体rel引起红蛋颜色和胚胎死亡的分子基础
昆虫卵的颜色和孵化率会影响个体和种群的生存。然而，很少有遗传基因位点被记录到影响这两种性状，并且参与调节这两种性状的基因尚不清楚。家蚕隐性突变体re l显示出红色的蛋色和胚胎死亡率。我们研究了re l表型形成的分子基础。通过遗传分析、基因筛选和测序，我们发现两个密切相关的基因BGIBMGA003497 ( Bm-re ) 和BGIBMGA003697 ( BmSema1a ) 分别控制鸡蛋颜色和胚胎死亡率。Bm-re 的六个碱基对基因在其开放阅读框中被删除，并且BmSema1a在突变体re l 中以异常低的水平表达。BmSema1a基因功能验证使用 RNA 干扰和成簇随机散布回文重复序列 (CRISPR)/CRISPR 相关蛋白 9 进行。BmSema1a基因缺陷会导致家蚕胚胎死亡。该研究揭示了家蚕re l突变体形成的分子基础，并表明Sema1a基因对昆虫胚胎发育至关重要。
更新日期：2020-09-11
中文翻译：
蚕突变体rel引起红蛋颜色和胚胎死亡的分子基础
昆虫卵的颜色和孵化率会影响个体和种群的生存。然而，很少有遗传基因位点被记录到影响这两种性状，并且参与调节这两种性状的基因尚不清楚。家蚕隐性突变体re l显示出红色的蛋色和胚胎死亡率。我们研究了re l表型形成的分子基础。通过遗传分析、基因筛选和测序，我们发现两个密切相关的基因BGIBMGA003497 ( Bm-re ) 和BGIBMGA003697 ( BmSema1a ) 分别控制鸡蛋颜色和胚胎死亡率。Bm-re 的六个碱基对基因在其开放阅读框中被删除，并且BmSema1a在突变体re l 中以异常低的水平表达。BmSema1a基因功能验证使用 RNA 干扰和成簇随机散布回文重复序列 (CRISPR)/CRISPR 相关蛋白 9 进行。BmSema1a基因缺陷会导致家蚕胚胎死亡。该研究揭示了家蚕re l突变体形成的分子基础，并表明Sema1a基因对昆虫胚胎发育至关重要。
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