Simultaneous Visualization of Wet Cells and Nanostructured Biomaterials in SEM using Ionic Liquids,ChemBioChem

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Simultaneous Visualization of Wet Cells and Nanostructured Biomaterials in SEM using Ionic Liquids
ChemBioChem ( IF 2.6 ) Pub Date : 2020-09-11 , DOI: 10.1002/cbic.202000552
Bryan E J Lee ₁ , Liza-Anastasia DiCecco ₂ , Hourieh Exir _{3,

4} , Arnaud Weck _{3,

4,

5} , Kyla N Sask _{1,

2} , Kathryn Grandfield _{1,

2}

Affiliation

Imaging cells with ionic liquids: Hydrated, unfixed mammalian cells adhered to laser‐modified nanostructured titanium substrates were observed by SEM after specialized ionic liquid treatment. The rapid and easy protocol using 1‐ethyl‐3‐methylimidazolium tetrafluoroborate as an ionic liquid, with dilution in culture medium, fulfills a niche need to study cellular interactions with biomaterials having submicron surface features.

中文翻译：

使用离子液体在 SEM 中同时可视化湿细胞和纳米结构生物材料

使用离子液体对细胞进行成像：经过专门的离子液体处理后，通过 SEM 观察粘附在激光改性纳米结构钛基底上的水合未固定哺乳动物细胞。使用 1-乙基-3-甲基咪唑鎓四氟硼酸盐作为离子液体并在培养基中稀释的快速简便方案满足了研究细胞与具有亚微米表面特征的生物材料相互作用的利基需求。

更新日期：2020-09-11

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