A 12‐week feeding trial was conducted to investigate the effects of dietary daidzein on the intestinal development and growth performance of turbot subjected to soybean meal. Three isonitrogenous and isolipidic diets with 520 g/kg crude protein and 100 g/kg crude lipid were formulated: fish meal diet (FM), FM replaced by 400 g/kg soybean meal protein diet (SBM) and SBM supplemented with 40 mg/kg daidzein diet (DAID). Compared with SBM, DAID improved the growth performance of turbot. The normal and healthy distal intestinal appearance was observed in DAID group, which is similar to FM group, while the typical enteritis was observed in SBM group. Fish fed DAID had higher intestosomatic index, relative gut length and absorptive surface than fish fed SBM. Compared with SBM, DAID significantly increased the expression of Wingless‐int (Wnt) 10b, β‐catenin, c‐myc and cyclin D1, and decreased the glycogen synthase kinase‐3 beta (GSK‐3β) expression. The transcriptome analysis between DAID and SBM showed that daidzein tended to promote cell proliferation, up‐regulate the genes enriched in pathways such as cell cycle, oocyte meiosis and progesterone‐mediated oocyte maturation. Therefore, the current study showed that dietary daidzein improved intestinal development of turbot, which might be related to the activation of Wnt/β‐catenin signalling pathway.

中文翻译：

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膳食大豆黄酮可改善豆turbo幼鱼肠的发育。（Scophthalmus maximus L.）

进行了为期12周的喂养试验，以研究膳食大豆苷元对受豆粕影响的大菱the肠道发育和生长性能的影响。配制了三种含氮520 g / kg粗蛋白和100 g / kg粗脂质的等氮和异脂饮食：鱼粉日粮（FM），以400 g / kg豆粕蛋白质日粮（SBM）代替FM和辅以40 mg / kg豆粕日粮公斤大豆黄酮饮食（DAID）。与SBM相比，DAID改善了大菱t的生长性能。DAID组观察到正常和健康的远端肠道外观，类似于FM组，而SBM组观察到典型的肠炎。饲喂DAID的鱼比饲喂SBM的鱼具有更高的肠道活性指数，相对肠道长度和吸收表面。与SBM相比，DAID显着增加了SID的表达Wingless-int（Wnt）10b，β-catenin，c-myc和cyclin D1并降低了糖原合酶激酶-3β（GSK-3β）的表达。DAID和SBM之间的转录组分析表明，大豆苷元倾向于促进细胞增殖，上调在细胞周期，卵母细胞减数分裂和孕酮介导的卵母细胞成熟等途径中富集的基因。因此，当前的研究表明，膳食黄豆苷元能改善大菱t的肠道发育，这可能与Wnt /β-catenin信号通路的激活有关。