Mycoplasma hyorhinis is a swine pathogen bacterium, which causes significant economic losses. The infection spreads through direct contact between the animals. Powerful genotyping methods like PCR based multi-locus sequence typing (MLST) and multiple-locus variable-number tandem-repeat analysis (MLVA) are necessary to monitor the infections and to conduct epidemiological investigations; hence supporting the control of the disease. The aims of the present study were to examine M. hyorhinis isolates originating mainly from Hungary with MLST and MLVA developed in the study, and to compare the results of the two typing methods.

To characterize 39 M. hyorhinis isolates and the type strain (NCTC 10,130), six house-keeping genes were selected for MLST and six tandem-repeat regions were chosen for MLVA. We were able to differentiate 31 sequence types and 37 genotypes within the 40 analyzed isolates by the MLST and the MLVA, respectively. With the combination of the two newly developed assays all examined isolates were distinguished with the exception of the ones originating from the same animal. The developed MLST assay provided a robust and high resolution phylogenetic tree, while the MLVA system is suitable for the differentiation of closely related isolates from the same farm, hence the assay is appropriate for epidemiologic studies.

猪支原体是一种猪病原菌，会造成重大的经济损失。感染通过动物之间的直接接触传播。强大的基因分型方法，例如基于PCR的多位点序列分型（MLST）和多位点可变数串联重复分析（MLVA），对于监测感染和进行流行病学调查至关重要。因此支持控制疾病。本研究的目的是用研究中开发的MLST和MLVA来检查主要来自匈牙利的M. hyorhinis分离株，并比较两种分型方法的结果。

为了表征39. M. hyorhinis分离株和类型菌株（NCTC 10,130），为MLST选择了6个持家基因，为MLVA选择了6个串联重复区。我们能够通过MLST和MLVA分别区分40种分析菌株中的31种序列类型和37种基因型。结合两种最新开发的测定法，除了源自同一动物的分离株外，所有被检出的分离株均得以区分。发达的MLST分析提供了鲁棒且高分辨率的系统发育树，而MLVA系统适用于区分同一农场中密切相关的分离株，因此该分析适合流行病学研究。