Aflatoxin B₁ (AFB₁) is one of the most common contaminants of poultry feed and has been linked to adverse effects on animal health and productivity. In this study, the degradation of AFB₁ was studied with cell-free extracts (CFE) of Trametes versicolor and Bacillus subtilis using High-Performance Liquid chromatography (HPLC). CFE from B. subtilis and T. versicolor gave 60% and 34% of AFB₁ degradation respectively, while heat-inactivated extracts showed no degradation. By-products obtained at the end of AFB₁ degradation were analyzed by Liquid Chromatography with tandem mass spectrometry (LC-MS/MS). After 96 h of incubation, by-products with lower m/z values were obtained with CFE from B. subtilis as compared to that from T. versicolor, indicating a higher degradation efficiency of the former. Additionally, the detection of a by-product which could correspond to AFB1-8,9 dihydrodiol - a less toxic derivative of AFB₁ - after 72 and 96 h of incubation with CFE from B. subtilis, could indicate the simultaneous detoxification along with degradation of AFB₁ by B. subtilis CFE.

黄曲霉毒素B ₁（AFB ₁）是家禽饲料中最常见的污染物之一，与对动物健康和生产力的不利影响有关。在这项研究中，使用高效液相色谱法（HPLC），用云芝Trametes和枯草芽孢杆菌的无细胞提取物（CFE）研究了AFB ₁的降解。枯草芽孢杆菌和杂色小麦的CFE分别使AFB ₁降解60％和34％，而热灭活提取物则无降解。AFB ₁结束时获得的副产物通过液相色谱-串联质谱（LC-MS / MS）分析降解。温育96小时后，副产物具有较低米/ ž用CFE从获得的值的枯草芽孢杆菌相比于从T.云芝，表明前者的更高的降解效率。此外，在与枯草芽孢杆菌CFE孵育72和96小时后，检测到可能对应于AFB1-8,9二氢二醇（一种毒性较小的AFB ₁衍生物）的副产物，可能表明同时进行了解毒和降解枯草芽孢杆菌CFE对AFB ₁的检测。