Current outbreaks associated with drug-resistant clinical strains are demanding for the development of broad-spectrum antibacterial agents. The bactericidal materials should be eco-friendly, economical and effective to suppress bacterial growth. Thus, in this work, diameter controlled spherical Cu_core-Ag_shell nanoparticles (Ag@CuNPs) with diameter ranging from 70 to 100 nm by one-step co-reduction approach were designed and synthesized. The Ag@CuNPs were homogenous, stable, and positively charged. The 70 nm Ag@CuNPs showed a consistent and regular Ag shielding. We observed the 100 nm Ag@CuNPs achieved symmetrical doped Ag clusters on the Cu core surface. We used Gram-positive and Gram-negative models strains to test the wide-spectrum antibacterial activity. The Ag@CuNPs showed detrimental microbial viability in a dose-dependent manner; however, 70 nm Ag@CuNPs were superior to those of 100 nm Ag@CuNPs. Initially, Ag@CuNPs attached and translocated the membrane surface resulting in bacterial eradication. Our analyses exhibited that antibacterial mechanism was not governed by the bacterial genre, nonetheless, by cell type, morphology, growing ability and the NPs uptake capability. The Ag@CuNPs were highly tolerated by human fibroblasts, mainly by the use of starch as glucosidic capper and stabilizer, suggesting optimal biocompatibility and activity. The Ag@CuNPs open up a novel platform to study the potential action of bimetallic nanoparticles and their molecular role for biomedical, clinical, hospital and industrial-chemical applications.

当前与耐药性临床菌株有关的暴发对广谱抗菌剂的开发提出了要求。杀菌材料应是环保，经济和有效的抑制细菌生长的材料。因此，在这项工作中，直径可控的球形Cu_核-Ag_壳通过一步共还原法设计并合成了直径范围为70至100 nm的纳米颗粒（Ag @ CuNPs）。Ag @ CuNPs均匀，稳定且带正电。70 nm Ag @ CuNPs表现出一致且规则的Ag屏蔽。我们观察到100 nm Ag @ CuNPs在铜芯表面上实现了对称掺杂的Ag团簇。我们使用革兰氏阳性和革兰氏阴性模型菌株来测试广谱抗菌活性。Ag @ CuNPs表现出有害的微生物生存能力，呈剂量依赖性。然而，70 nm Ag @ CuNPs优于100 nm Ag @ CuNPs。最初，Ag @ CuNPs附着并转移到膜表面，导致细菌根除。我们的分析表明，抗菌机制不受细菌种类的控制，但受细胞类型，形态，生长能力和NPs吸收能力。Ag @ CuNPs被人成纤维细胞高度耐受，主要是通过使用淀粉作为糖苷封端剂和稳定剂，表明具有最佳的生物相容性和活性。Ag @ CuNPs为研究双金属纳米粒子的潜在作用及其在生物医学，临床，医院和工业化学应用中的分子作用提供了一个新的平台。