Many features that are appropriate for an ideal tissue engineered biomaterial are found in plant tissues. Hierarchically organized Bambusa vulgaris exhibits structural similarities to native bone, but the degradation of cellulose that is the main component of the plant cell wall is a challenge. In this study, Bamboo stem was subjected to decellularization followed by a chemical oxidation process (treated with sodium periodate) to enhance biocompatibility and biodegradation. The crystallinity of oxidised plant scaffolds was reduced, resulting in lower mechanical strength. In contrast, hydrophilicity was enhanced in those scaffolds. In vitro studies demonstrated better mesenchymal stem cell adhesion, viability, and osteogenic differentiation on oxidized scaffolds. Those scaffolds also induced angiogenesis, biocompatibility, and biodegradation when implanted subcutaneously in vivo. Hence, the present study demonstrated the usefulness of “oxidized decellularized plant” as bone scaffold for non-load-bearing applications.

在植物组织中发现了许多适用于理想组织工程生物材料的特征。具有层次结构的寻常型班布沙草与天然骨具有相似的结构，但是纤维素的降解是植物细胞壁的主要组成部分，这是一个挑战。在这项研究中，竹茎经过脱细胞处理，然后进行化学氧化处理（用高碘酸钠处理），以增强生物相容性和生物降解性。氧化的植物支架的结晶度降低，导致较低的机械强度。相反，在那些支架中亲水性增强。体外研究表明，在氧化支架上，间充质干细胞具有更好的粘附性，生存能力和成骨分化能力。当在体内皮下植入时，那些支架还诱导血管生成，生物相容性和生物降解。因此，本研究证明了“氧化脱细胞植物”作为骨支架在非承重应用中的有用性。