As discovery research organizations push more molecules and new modalities through their company pipelines, there comes a need to widen purification development and production bandwidth by increasing automation and throughput. Continuous processing technologies have the unique property of reducing manufacturing floor space and reducing costs. We can speed development and production by implementing automation and continuous process technologies early in discovery research. Here we describe an automated continuous instrument made up of an ÄKTA™ pcc for initial capture by protein A, an ÄKTA pure 150 retrofitted to automatically condition protein A eluate, and a second ÄKTA pure 150 built for flow-through anion exchange chromatography. The continuous instrument we have designed and built recirculates protein A eluate from the ÄKTA pcc in a closed loop while signals from the pH and conductivity meters direct addition of titrant for accurate and precise adjustments to the pH and salt concentration. The instrument is run without user intervention and can be used continuously for production or for development as a tool for screening running conditions on the anion exchange step.

随着发现研究组织通过其公司管道推动更多的分子和新模式，需要通过提高自动化和吞吐量来扩大纯化开发和生产带宽。连续加工技术具有减少制造占地面积和降低成本的独特特性。我们可以通过在发现研究早期实施自动化和连续工艺技术来加快开发和生产。在这里，我们描述了一种自动化连续仪器，由用于蛋白质 A 初始捕获的 äKTA™ pcc、经过改造以自动调节蛋白质 A 洗脱液的 äKTA pure 150 和用于流通式阴离子交换色谱的第二个 äKTA pure 150 组成。我们设计和制造的连续仪器在闭环中再循环 AKTA pcc 的蛋白 A 洗脱液，同时来自 pH 和电导计的信号直接添加滴定剂，以准确、精确地调节 pH 和盐浓度。该仪器的运行无需用户干预，可连续用于生产或开发，作为筛选阴离子交换步骤运行条件的工具。