1,3-Propanediol (1,3-PDO) is a valuable compound with a large potential market in many industries. This study aimed to evaluate the abilities of the Psychrophile-based Simple bioCatalyst (PSCat) reaction system to biosynthesize 1,3-PDO. This biocatalyst has a potential platform that replaces the chemical-based production counterparts. The two genes involved in the metabolic pathway were expressed both individually and together in the psychrophilic host bacterium. The intracellular metabolic flux was deactivated using heat treatment, at 45 °C for 15 min. After individual gene expression (25.0 mM), 1,3-PDO productivity of the cells increased by approximately 2.5 times, in comparison to when genes were expressed together (10.2 mM). Productivity was boosted (31.1 mM) when the cofactor regeneration system was activated in the biocatalyst. Hence, both the ability of individual gene expression and the cofactor regeneration system were verified in the PSCat approach. Nonetheless, further research is necessary to develop and optimize this process for industrial production.

1,3-丙二醇 (1,3-PDO) 是一种有价值的化合物，在许多行业具有巨大的潜在市场。本研究旨在评估基于嗜冷剂的简单生物催化剂 (PSCat) 反应系统生物合成 1,3-PDO 的能力。这种生物催化剂有一个潜在的平台，可以取代基于化学的生产对应物。参与代谢途径的两个基因在嗜冷宿主细菌中单独和一起表达。使用热处理在 45°C 下 15 分钟使细胞内代谢通量失活。与基因一起表达时 (10.2 mM) 相比，单个基因表达 (25.0 mM) 后，细胞的 1,3-PDO 生产力增加了约 2.5 倍。当辅因子再生系统在生物催化剂中被激活时，生产力得到提高（31.1 mM）。因此，在 PSCat 方法中验证了单个基因表达的能力和辅因子再生系统。尽管如此，还需要进一步的研究来开发和优化这一工业生产过程。