14-3-3 proteins are widespread in animals, but their functions and mechanisms remain poorly defined. Here we clearly demonstrate that 14-3-3 β/α-A is a newly identified PGN-binding protein present abundantly in the eggs/embryos of zebrafish. We also show that recombinant 14-3-3 β/α-A acts as a pattern recognition receptor capable of identifying the bacterial signature molecule PGN, binding the bacteria, and functions as an antibacterial effector molecule directly killing the bacteria. Importantly, microinjection of r14-3-3 β/α-A into early embryos significantly enhanced the resistance of the embryos against pathogenic A. hydrophila challenge, and this enhanced bacterial resistance was markedly reduced by co-injection of anti-14-3-3 β/α-A antibody. Collectively, these results indicate that 14-3-3 β/α-A is a maternal PGN-binding protein that can protect the early embryos of zebrafish against pathogenic attacks, a novel role assigned to 14-3-3 β/α-A proteins. This work also provides new insights into 14-3-3 proteins that are widely distributed in various animals.

14-3-3 蛋白在动物中广泛存在，但其功能和机制仍不清楚。在这里，我们清楚地证明 14-3-3 β/α-A 是一种新发现的 PGN 结合蛋白，大量存在于斑马鱼的卵子/胚胎中。我们还表明，重组 14-3-3 β/α-A 作为一种模式识别受体，能够识别细菌特征分子 PGN，与细菌结合，并作为一种直接杀死细菌的抗菌效应分子发挥作用。重要的是，将 r14-3-3 β/α-A 显微注射到早期胚胎中显着增强了胚胎对致病性 A.hydrophila的抵抗力挑战，并且通过共同注射抗 14-3-3 β/α-A 抗体显着降低了这种增强的细菌抗性。总的来说，这些结果表明 14-3-3 β/α-A 是一种母体 PGN 结合蛋白，可以保护斑马鱼的早期胚胎免受病原体攻击，这是分配给 14-3-3 β/α-A 的新角色蛋白质。这项工作还为广泛分布在各种动物中的 14-3-3 蛋白质提供了新的见解。