Abstract Paper-based point-of-care (POC) devices have attracted tremendous attention because of low-cost, simple fabrication, and portability. LAMP-based POC platform comprises a paper substrate on which the DNA amplification reaction occurs. The quality of the paper substrate plays a critical role in performing an efficient LAMP reaction and for effective visualization of the amplification result. The major objective of the current work is to replace the frequently utilized nitrocellulose (NC) membrane by a cost-effective and high quality paper membrane viz. Whatman filter paper (WF) for rapid detection of E. coli via LAMP reaction. Isolated DNA is added on the paper membranes, which undergo LAMP reaction. The effect of initial DNA concentration and time on LAMP amplification reaction rate is analyzed on paper membranes via image analysis for the first time. Overall study inferred that WF is suitable for effective naked-eye detection and greater extent of LAMP reaction (generation of 19 968 DNA copies).

中文翻译：

---

在床旁诊断试剂盒中为环介导等温 DNA 扩增反应 (LAMP) 选择合适的纸膜 - 实验和 CFD 分析

摘要 基于纸张的即时护理 (POC) 设备因其低成本、制造简单和便携性而引起了极大的关注。基于 LAMP 的 POC 平台包括一个纸基材，DNA 扩增反应发生在纸基材上。纸基材的质量在执行有效的 LAMP 反应和扩增结果的有效可视化方面起着至关重要的作用。当前工作的主要目标是用具有成本效益的高质量纸膜代替经常使用的硝化纤维 (NC) 膜，即。Whatman 滤纸 (WF)，用于通过 LAMP 反应快速检测大肠杆菌。将分离的 DNA 添加到纸膜上，进行 LAMP 反应。首次通过图像分析在纸膜上分析了初始 DNA 浓度和时间对 LAMP 扩增反应速率的影响。整体研究推断，WF 适用于有效的肉眼检测和更大程度的 LAMP 反应（产生 19 968 个 DNA 拷贝）。