The aim of this study was to investigate the relationship between serum metabolomic biomarkers and brain in vivo magnetic resonance spectroscopy (MRS) biomarkers in patients with Parkinson’s disease (PD) as well as to investigate compound concentration changes by comparing the results with healthy control subjects. Univariate statistical analysis of the serum showed significant differences in the levels of phenylalanine, tyrosine, lysine, glutamine, glutamate, acetone, acetate, 3-hydroxybutyrate, and 1-monoacylglycerol (1-MAG) between the PD patient group and the control group. Orthogonal partial least squares discriminant analysis showed significantly different compound concentrations of acetate, 3-hydroxybutyrate, glutamine, tyrosine, 1-MAG and testosterone. In vivo MRS of the putamen showed significantly higher concentrations of glutamine/glutamate complex and glutamine in patients with PD in comparison to control subjects. Following disrupted metabolic pathways in patients with PD were identified: dopamine synthesis, steroid hormone biosynthesis, fatty acid biosynthesis, the synthesis and degradation of ketone bodies, the metabolism of pyruvate, arginine, proline, alanine, aspartate, glutamate, tyrosine and phenylalanine. The obtained results may indicate changes in neurotransmission, disturbances in energy production and an altered cell membrane structure.

本研究的目的是研究帕金森病 (PD) 患者血清代谢组学生物标志物与脑体内磁共振波谱 (MRS) 生物标志物之间的关系，并通过将结果与健康对照受试者进行比较来研究化合物浓度的变化。血清的单变量统计分析显示，PD患者组与对照组之间苯丙氨酸、酪氨酸、赖氨酸、谷氨酰胺、谷氨酸、丙酮、醋酸、3-羟基丁酸酯和1-单酰基甘油（1-MAG）水平存在显着差异。正交偏最小二乘判别分析显示乙酸盐、3-羟基丁酸盐、谷氨酰胺、酪氨酸、1-MAG 和睾酮的化合物浓度显着不同。体内与对照组相比，PD 患者壳核的 MRS 显示出明显更高浓度的谷氨酰胺/谷氨酸复合物和谷氨酰胺。确定了 PD 患者的以下代谢途径中断：多巴胺合成、类固醇激素生物合成、脂肪酸生物合成、酮体的合成和降解、丙酮酸、精氨酸、脯氨酸、丙氨酸、天冬氨酸、谷氨酸、酪氨酸和苯丙氨酸的代谢。获得的结果可能表明神经传递的变化、能量产生的干扰和细胞膜结构的改变。