Electrophysiological study that records the action potential of cardiomyocyte served as excellent tool to explore cardiology and neuroscience, disease investigation and pharmacological screening. Advances of micro/nanotechnologies promote the development of three-dimensional (3D) nanodevices to record high-quality intracellular recordings by various perforation approaches of cells, however, the complicated fabrication processes limited their large-scale manufacture. In this work, a unique nanobranched microelectrode array (NBMEA) platform is developed to achieve high-quality intracellular recording of cultured cardiomyocytes in a minimally invasive manner. The NBMEA is consisting of high aspect ratio conductive nanobranches fabricated on patterned microelectrodes combining hydrothermal growth and standard microfabrication. The 3D structure of nanobranches enables the electrode to form tight coupling with cardiomyocytes to achieve the low voltage cell electroporation and high-quality intracellular recording. The recorded intracellular action potentials of cardiomyocytes by NBMEA exhibited significant enhancement on amplitude (~5 mV), signal-to-noise ratio (SNR) (~67.47 dB), recording duration (up to 105 min), and recording yield (69.5 ± 17.8%). This NBMEA platform is a promising and powerful tool for electrophysiology that opens up new opportunities for high-quality and stable intracellular recording of cardiomyocytes.

记录心肌细胞动作电位的电生理研究是探索心脏病学和神经科学，疾病调查和药理学筛选的极佳工具。微/纳米技术的进步促进了三维（3D）纳米器件的发展，以通过各种细胞穿孔方法来记录高质量的细胞内记录，但是，复杂的制造工艺限制了它们的大规模生产。在这项工作中，开发了独特的纳米支化微电极阵列（NBMEA）平台，以最小的侵入性方式实现了对培养的心肌细胞的高质量细胞内记录。NBMEA由结合水热生长和标准微细加工的图案化微电极上制造的高纵横比导电纳米分支组成。纳米支的3D结构使电极能够与心肌细胞紧密结合，从而实现低压细胞电穿孔和高质量的细胞内记录。NBMEA记录的心肌细胞胞内动作电位在幅度（〜5 mV），信噪比（SNR）（〜67.47 dB），记录持续时间（长达105分钟）和产量（69.5± 17.8％）。该NBMEA平台是一种有前途且功能强大的电生理工具，为高质量，稳定地记录心肌细胞开辟了新的机会。NBMEA记录的心肌细胞胞内动作电位在幅度（〜5 mV），信噪比（SNR）（〜67.47 dB），记录持续时间（长达105分钟）和产量（69.5± 17.8％）。该NBMEA平台是一种有前途且功能强大的电生理工具，为高质量，稳定地记录心肌细胞开辟了新的机会。NBMEA记录的心肌细胞内动作电位在幅度（〜5 mV），信噪比（SNR）（〜67.47 dB），记录持续时间（长达105分钟）和产量（69.5± 17.8％）。该NBMEA平台是一种有前途且功能强大的电生理工具，为高质量，稳定地记录心肌细胞开辟了新的机会。