New world Coral snakes comprise 82 species of medical importance distributed from southeastern United States to Argentina. In Colombia, Micrurus mipartitus and M. dumerilii are responsible for most coral snakebite accidents. Although infrequent, the severity of these envenomings, as well as the limited information available on the neutralizing coverage of commercially available antivenoms, underscores the need to perform studies to assess the cross-neutralizing ability of these life-saving immunobiologicals. In the present work, we evaluated the cross-recognition and neutralization ability of two equine therapeutic antivenoms: PROBIOL and SAC-ICP. PROBIOL antivenom showed cross-recognition towards both M. mipartitus and M. dumerilii venoms, with a significantly higher binding to the latter in both whole-venom ELISA and fractionated-venom immunoprofiling. In contrast, SAC-ICP antivenom cross-recognized M. dumerilii venom, but not that of M. mipartitus. Lethality of M. dumerilii venom was neutralized by both antivenoms, with a slightly higher potency for the SAC-ICP antivenom. However, the lethality of M. mipartitus venom was not neutralized by any of the two antivenoms. Results uncover the need to include M. mipartitus venom, or its most relevant toxins, in the production of coral snake antivenoms to be used in Colombia, to assure the neutralizing coverage for this species.

新世界珊瑚蛇包括从美国东南部到阿根廷分布的 82 种具有重要医学意义的物种。在哥伦比亚，Micrurus mipartitus和M. dumerilii是造成大多数珊瑚蛇咬伤事故的罪魁祸首。尽管不常见，但这些毒液的严重程度以及关于市售抗蛇毒血清的中和覆盖率的有限信息，强调需要进行研究以评估这些挽救生命的免疫生物学的交叉中和能力。在目前的工作中，我们评估了两种马治疗性抗蛇毒血清的交叉识别和中和能力：PROBIOL 和 SAC-ICP。PROBIOL 抗蛇毒血清显示出对M. mipartitus和M. dumerilii 的交叉识别毒液，在全毒液 ELISA 和分馏毒液免疫分析中与后者的结合明显更高。相比之下，SAC-ICP 抗蛇毒血清可以交叉识别M. dumerilii毒液，但不能交叉识别M. mipartitus 的毒液。M. dumerilii毒液的致死性被两种抗蛇毒血清中和，而 SAC-ICP 抗蛇毒血清的效力略高。然而，M. mipartitus毒液的杀伤力并没有被两种抗蛇毒血清中的任何一种中和。结果表明，在哥伦比亚使用的珊瑚蛇抗蛇毒血清的生产中需要包括M. mipartitus毒液或其最相关的毒素，以确保对该物种的中和覆盖。